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ID   PUCD5      preliminary; circular DNA; SYN; 13000 BP.
AC   ATCC37344;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pUCD5 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pSa734 from pSa151 & pBR322
RC   pUCD2 from pSa734
RC   pUCD4 from pUCD2 & linker
RC   pUCD9P from pUCD2
RC   pUCD9X from pUCD4
RC   pUCD3 from pSa152 & pHC79
RC   pUCD5 from pUCD3 & pUCD2
RA   Close T.J., Zaitlin D., Kado C.I.;
RT   "Design and development of amplifiable broad-host-range cloning
RT   vectors: analysis of the vir region of Agrobacterium tumefaciens
RT   plasmid pTiC58";
RL   Plasmid 12:111-118(1984).
CC   An amplifiable broad host range cosmid vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUCD5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSa SstII-SstII 13300bp, spec/kan genes
FT                   -> pSa152 13600bp
FT                   1. pSa152 HindIII 13600bp
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   HindIII-HindIII, lambda cos
FT                   -> pUCD3 15300bp
FT                   1. pUCD3 KpnI-SacII, lambda cos/pSa152
FT                   2. pUCD2 remove small KpnI-SacII, 13000bp
FT                   -> pUCD5 13000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-SacI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas pSa151"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;