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ID   PUFR034    preliminary; circular DNA; SYN; 8700 BP.
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AC   ATCC77122;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Broad host range/Xanthomonas/E.coli cosmid vector pUFR034 - incomp.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUFR001, pUFR002, pUFR003 from pLAFR3 & Tn5
RC   plasmids from pVK102 & Tn5
RC   plasmid from pUFA-704 & pKT210
RC   pUFR004 from pLAFR3 & pCGN565
RC   pUFR006 from pKT230 & pCGN565
RC   pUFR007 from pSa747 & M13tg131
RC   pUFR008 from pUFR004 & pUFR007
RC   pUFR009 from pUFR006 & pUFR007
RC   pUC4Kan from pUC4 & kan gene
RC   pUFR010 from pUFR008 & pUC4Kan
RC   pUFR011 from pUFR010 & pUC18
RC   pUFR026 from pUFR011 & pUCD550
RC   pUFR027 from pUFR026
RC   pUFR030 from pUFR011
RC   pUFR032 from pUFR030 & pHC79
RC   pUFR033 from pUFR032
RC   pUFR034 from pUFR033 & pUFR037
RA   DeFeyter R., Kado C.I., Gabriel D.W.;
RT   "Small, stable shuttle vectors for use in Xanthomonas";
RL   Gene 88:65-72(1990).
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CC   The order of the major features in this plasmid is:lacZalpha with MCS
CC   (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA
CC   (pUC4Kan) - mob(P) (pLAFR3, Mob+).
CC   Shuttle vector permitting visual detection of recombinants. (ATCC
CC   staff)
CC   Uses interruption of lacZalpha. (ATCC staff)
CC   The partition locus from Agrobacterium plasmid pTAR stabilizes the
CC   plasmid in X. citri and X. campestris pvs. campestris, vesicatoria,
CC   malvacearum, and translucens grown in broth culture.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8. (ATCC staff)
CC   Medium is 1799 LB plus neomycin (10 ug/ml).
CC   NM (pUFR034)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(broad host range)(E.coli)(Xanthomonas)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. parA locus
FT                   -> pUFR037
FT                   1. pUFR010 large BamHI-PvuII
FT                   BAL31 nuclease
FT                   2. pUC18 HaeII-HaeII, lacZ gene
FT                   BAL31 nuclease
FT                   -> pUFR011
FT                   1. pUFR011 remove BglII-Sau3A 2400bp
FT                   -> pUFR030 [BglII]
FT                   1. pUFR030 BglII
FT                   2. pHC79 BglII-BglII, lambda cos
FT                   -> pUFR032
FT                   1. pUFR032 BglII, Sp
FT                   fill in
FT                   -> pUFR033
FT                   1. pUFR033 BglII
FT                   2. pUFR037 BamHI-BamHI, parA locus
FT                   -> pUFR034 8700bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI Xanthomonas oriW"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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