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ID   PUK21      preliminary; circular DNA; SYN; 144 BP.
AC   M74307;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pUK21 - incomplete, MCS.
KW   cloning vector; MCS.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-144
RC   MCS from pMTL23 & pBluescript SK
RC   pUC21 from pUC118 & linker MCS
RC   pUK21 from pUC21 & Tn903
RC   pUC6S from MCS & pUC6; [pUC with no lacZ]
RC   pOK12 from pUC21 & Tn903 & pACYC177
RA   Vieira J., Messing J.;
RT   "New pUC-derived cloning vectors with different selectable markets
RT   and DNA replication origins";
RL   Gene 100:189-194(1991).
CC   NCBI gi: 209215
CC   NM (pUK21)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC21)(Tn903)
CC   BR (pUC21)(pOK12)(pUC6S)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC21 BspHI-BspHI, 3306bp-
FT                   \ pUC118 BspHI = 2003 3011 3116
FT                   fill in:fill in
FT                   2. Tn903 XhoI, kan gene
FT                   blunt end
FT                   ClaI, kan gene
FT                   blunt end
FT                   ?-? 975bp 1063..2038, #V00359
FT                   -> pUK21 3000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 144 BP; 32 A; 41 C; 39 G; 32 T; 0 other;
     atgaccatga ttacgccact agtccgaggc ctcgagatct atcgatgcat gccatggtac
     ccgggagctc gaattcgaag cttctgcaga cgcgtcgacg tcatatggat ccgatatcgc
     cgtggcggcc gctctagaac tagt