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ID   PUR18      preliminary; circular DNA; SYN; 5500 BP.
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AC   ATCC77297;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Schizosaccharomyces/E.coli plasmid vector pUR18 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUR18 from pUC18 & pFL201.2
RC   pUR19 from pUC19 & pFL201.2
RC   pUR18N from pUR18
RC   pUR19N from pUR19
RC   pUC-DIS from pUC18
RC   pUD18 from ura4
RA   Barbet N., Muriel W.J., Carr A.M.;
RT   "Versatile shuttle vectors and genomic libraries for use with
RT   Schizosaccharomyces pombe";
RL   Gene 114:59-66(1992).
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RN   [2]
RC   pARS727, pARS744, pARS745 from M13mp19 & S. pombe genomic DNA, ARS
RC   pARS747, pARS756, pARS766 from M13mp19 & S. pombe genomic DNA, ARS
RC   pARS767, pARS772 from M13mp19 & S. pombe genomic DNA, ARS
RC   pFL201.2 from pFL20, ARS
RA   Maundrel K., Hutchinson A., Shall S.;
RT   "Sequence analysis of ura4 elements in fission yeast";
RL   EMBO J. 7:2203-2209(1988).
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CC   The order of the major features in this plasmid is:ars1 - ura4 -
CC   HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR.
CC   Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a
CC   1.2 kb ClaI fragment containing ars1 into the NdeI site modified with
CC   a ClaI linker.
CC   Shuttle vector permitting visual detection of recombinants by
CC   beta-galactosidase alpha complementation.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pUR18)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(Schizosaccharomyces pombe)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pBR322-8 5527bp
FT                   1. pBR322-8 5527bp, URA3 gene
FT                   2. S. pombe, ARS
FT                   -> pFL20
FT                   1. pFL20 EcoRI-EcoRI 1200bp, S.pombe ARS
FT                   -> pFL201.2
FT                   1. pUC18 NdeI 2686bp 185..185
FT                   2. pFL201.2 ClaI-ClaI 1200bp, S.pombe ARS1
FT                   3. S. pombe ClaI-ClaI 1700bp, URA4 gene
FT                   -> pUR18 5500bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-PstI-SalI-AccI-
FT                   HincII-BamHI-SmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA4 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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