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ID   PUT330     preliminary; circular DNA; SYN; 1 BP.
AC   M32023; M32024; M32025; M32026; M32027;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pUT330 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pUT71 from pBG131 & pJV160, ENO1 gene
RC   pUT330 from pFL44L & pUT302, ble gene
RC   pUT331 from pUT330 & pSP64-p1, TEF1 gene
RC   pUT332 from pFL44L & pUT331
RC   pUT350 from pFL44L & pUT71
RC   pUT333 from pUT330 & pUT18
RA   Gatignol A., Dassain M., Tiraby G.;
RT   "Cloning of Saccharomyces cerevisiae promoters using a probe vector
RT   based on phleomycin resistance";
RL   Gene 91:35-41(1990).
RN   [2]
RC   pUT9 from pKC7 & linker
RC   pUT14 from pUT9 & linker
RC   pUT18 from pUT14 & pJRD158
RC   pUT67 from pEX-2 & pUT9
RC   pUT302 from pEX-2 & pUT9, ble gene
RC   pUT311 from pUT302 & pUT18
RC   pUT303, pUT304, pUT305, pUT308, pUT312, pUT313 from pUT302
RA   Gatignol A., Baron M., Tiraby G.;
RT   "Phleomycin resistance encoded by the ble gene from transposon Tn
RT   5 as a dominant selectable marker in Saccharomyces cerevisiae";
RL   Mol. Gen. Genet. 207:342-348(1987).
RN   [3]
RC   pSP64-p1 from pSP64 & TEF1 gene
RA   Buhler J.M.;
RT   ;
RL   Unpublished (1990).
RN   [4]
RC   pcEG-10 from endoglucase I gene
RC   pMAC101 from pAC1
RC   pUC8-h from pUC8 & Trichoderma reesei endoglucase I gene
RC   plasmid from pUC8-h & M13mp19
RC   plasmid2 from plasmid & pcEG-10
RC   pJV160 from plasmid2 & pMAC101
RA   Van Arsdell J.N., Kwok S., Scheickart V.L., Ladner M.B.,
RA   Gelfand D.H., Innis M.A.;
RT   "Cloning, characterization and expression in Saccharomyces
RT   cerevisiae of endoglucase I from Trichoderma reesei";
RL   Biotechnology 5:60-64(1987).
RN   [5]
RC   pEX-2 from pBR322 & yeast 2 micron & CYC1 gene & URA3 gene
RA   Ernst J.F., Chan R.K.;
RT   "Characterization of Saccharomyces cerevisiae mutants supersensitive
RT   to aminoglycoside antibiotics";
RL   J. Bacteriol. 163:8-14(1985).
CC   new sequence. in GenBank. promoters.
CC   NM (pUT330)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pFL44)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast, ARS1
FT                   3. yeast, CYC1 terminator/promoter
FT                   4. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   5. yeast 2 micron, ori
FT                   -> pEX-2 9300bp
FT                   1. pKC7 SphI 5835bp 875..875, 3' kan gene
FT                   BAL31 nuclease
FT                   BglII linker 6bp agatct
FT                   -> plasmid 5100bp
FT                   1. plasmid XhoI 5100bp 1623..1623, 3' Tn5 phleo gene
FT                   \ #A00223/#A00224
FT                   BamHI linker 6bp ggatcc
FT                   remove small BamHI-BamHI 225bp 1623..1848, 4800bp
FT                   -> pUT9 4800bp [no kan gene]
FT                   1. pEX-2 AsuII-BamHI, Tn5 phleo gene
FT                   \ #A00223/#A00224
FT                   fill in:
FT                   2. pUT9 EcoRI-BamHI, ble gene, pKC7 ?..1848
FT                   fill in:
FT                   -> pUT302 9700bp [AsuII, EcoRI]
FT                   1. pFL44L remove SalI-HindIII 2496bp 4009..6063..442
FT                   \ lacZ/3567bp
FT                   2. pUT302 XhoI-HindIII 1800bp, Tn5 ble/CYC1 prom/term
FT                   -> pUT330 5400bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;