ID   PVK100     preliminary; circular DNA; SYN; 23000 BP.
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AC   ATCC37156;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pVK100 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pVK100 from pRK290 & pHK17
RC   pVK101 from pVK100
RC   pVK102 from pVK100
RC   pVK200 series from pVK102 & pTiA6
RC   pVK261 from pVK102 & pTiA6
RA   Knauf V.C., Nester E.W.;
RT   "Wide host range cloning vectors: a cosmid clone bank of an
RT   Agrobacterium Ti plasmid";
RL   Plasmid 8:45-54(1982).
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RN   [2]
RC   pHK17 from pRK2501 & pHC79
RC   pHK111, pHK121, pHK120, pHK210 from pHK17 & pTiA6
RC   pTiA6::Tn5 from pTiA6 & Tn5
RA   Klee H., Gordon M.P., Nester E.W.;
RT   "Complementation analysis of Agrobacterium tumefaciens Ti plasmid
RT   mutations affecting oncogenicity";
RL   J. Bacteriol. 150:327-331(1982).
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RN   [3]
RC   pTiB6-806 from Agrobacterium Ti octopine plasmid
RC   pTiA6 from Agrobacterium Ti octopine plasmid
RC   pTiACH5 from Agrobacterium Ti octopine plasmid
RC   pTiT37 from Agrobacterium Ti nopaline plasmid
RC   pTiC58 from Agrobacterium Ti nopaline plasmid
RA   Nester E.W., Kosuge T.;
RT   "Plasmids specifying plant hyperplasias";
RL   Annu. Rev. Microbiol. 35:531-565(1981).
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RN   [4]
RC   from pVK102 & OpMNPV
RA   Chen D.D., Nesson M.H., Rohrmann G.F., Beaudreau G.S.;
RT   "The genome of the multicapsid baculovirus of Orgyia pseudosugata:
RT   restriction map and analysis of two sets of GC-rich repeated
RT   sequences";
RL   J. Gen. Virol. 69:1375-1381(1988).
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CC   A cosmid, broad host range cloning vector. (ATCC staff)
CC   Mobilization by the helper plasmid pRK2013 (ATCC 37159).
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pVK100)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RK2, oriT/tet gene
FT                   -> pRK248 10000bp
FT                   1. pRK248 10000bp
FT                   2. E. coli 1100bp, kan gene
FT                   -> pRK2501 11100bp
FT                   1. pRK2501 BglII 11100bp
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pHK17 12800bp
FT                   1. RK2
FT                   -> pRK290 20000bp
FT                   1. pRK290 SalI-EcoRI, trfA/trfB genes
FT                   2. pHK17 SalI-EcoRI, oriV
FT                   -> pVK100 23000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SalI-HindIII-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//