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ID   PWR2       preliminary; circular DNA; SYN; 2183 BP.
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AC   IG8046;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pWR2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pWR450 from pUC13
RC   pWR590 from pUC13 & pBGP120
RA   Guo L., Stepien P.P., Tso J.Y., Brousseau R., Narang S.,
RA   Thomas D.Y., Wu R.;
RT   "Synthesis of human insulin gene VIII. Construction of expression
RT   vectors for fused proinsulin production in Escherichia coli";
RL   Gene 29:251-254(1984).
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RN   [2]
RC   pWR1 from pUR222
RC   pWR2 from pWR1 & linker
RC   pWR3, pWR4, pWR5 from pWR2
RA   Guo L.H., Wu R.;
RT   "Exonuclease III: use for sequence analysis and specific deletion
RT   of nucelotides";
RL   Meth. Enzymol. 100:60-96(1983).
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RN   [3]
RC   pWR450 from pUC13
RC   pWR590 from pUC13 & pBGP120
RA   Wu R., Guo L.H., Stepien P.P., Shen T.J., Tso J.Y., Brousseau R.,
RA   Narang S.A., Thomas D.Y.;
RT   "Expression vectors for fused proinsulin production in E. coli and
RT   yeast";
RL   Symp. International Congress of Genetics, New Delhi 0:0-0(1984).
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CC   NM (pWR2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR222 PvuII 2712bp 1742..1742,
FT                   \ 5' to lac promoter/may be 2037..2037
FT                   BAL31 nuclease, remove 530bp/2180bp
FT                   -> pWR1 2180bp [unique PvuII]
FT                   1. pWR1 remove EcoRI-BamHI 9bp, MCS/pUR222 1830..1839
FT                   \ 2171bp
FT                   Klenow
FT                   HindIII linker 8bp caagcttg
FT                   -> pWR2 2183bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="SIT RsaI"
FT   misc_binding    0..0
FT                   /note="SIT XmnI"
FT   promoter        0..0
FT                   /note="GEN E. coli lac promoter/operator"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
FT   misc_binding    0..0
FT                   /note="MCS PstI-SalI-BamHI-HindIII-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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