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ID   PY3        preliminary; circular DNA; SYN; 5870 BP.
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AC   ATCC37322;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pY3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pMLTR-I from pMLTR
RC   pY3 from pMLTR-I & pLG88
RC   [pYN from HSV thymidine kinase gene & pY3, hygromycin B resistance]
RA   Blochinger K., Diggelmann H.;
RT   "Hygromycin B phosphotransferase as a selectable marker for DNA
RT   transfer experiments with higher eucaryotic cells";
RL   Mol. Cell. Biol. 4:2929-2931(1984).
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RN   [2]
RC   from Charon 4A & rat c-mos gene
RC   pMSV-31 from M-MSV transforming sequences
RC   pCS-1 from mouse c-mos gene
RC   pMLTR from Moloney murine sarcoma virus M-MSV
RA   Van Der Hoorn F.A., Hulsebos E., Berns A.J., Bloemers H.P.;
RT   "Molecularly cloned c-mos (rat) is biologically active";
RL   EMBO J. 1:1313-1317(1982).
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CC   Plasmid vector allowing for direct selection for hygromycin B
CC   resistance after transfection of eukaryotic cell lines.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.9, 0.9; KpnI--6.0; PstI--4.8, 1.2. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pY3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M-MSV EcoRI-EcoRI, M-MSV LTR
FT                   2. pBR322 EcoRI 4361bp 4360..4360
FT                   3. M-MSV PstI-PstI, M-MSV LTR
FT                   4. pBR322 PstI 4361bp 3612..3612
FT                   -> pMLTR
FT                   1. pMLTR remove SmaI-SmaI, MoSV LTR
FT                   -> pMLTR-I
FT                   1. pEX-2 5787bp
FT                   2. yeast, CYC1 promoter
FT                   3. E. coli 1141bp, hyg gene
FT                   -> pLG88 7000bp
FT                   1. pMLTR-I SmaI
FT                   2. pLG88 BamHI-BamHI 1150bp, hyg gene
FT                   blunt end:blunt end
FT                   -> pY3 5870bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SstI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT vertebrate hygromycin phosphotransferase
FT                   gene; hygromycin resistance gene (hyg)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   promoter        0..0
FT                   /note="PRO Mo-SV LTR"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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