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ID   PYADE4     preliminary; circular DNA; SYN; 6037 BP.
XX
AC   M74016;
XX
DT   01-NOV-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pYADE4 - incomplete, TRP1AMPr.
XX
KW   cloning vector; promoter; beta-lactamase gene; TRP1 gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6037
RC   pYADE4 from TRP1 gene & AMP gene
RC   pYADH2 from AMP gene & TRP1 gene & ADH2 gene & CYC1 gene
RC   pYSVE2 from TRP1 gene & AMP gene
RA   Brunelli J.P., Pall M.L.;
RT   "A series of yeast vectors for expression of cDNAs and other DNA
RT   sequences";
RL   Yeast 9:1299-1308(1993).
XX
RN   [2]
RP   1-6037
RC   pYADE4
RA   Pall M.L.;
RT   ;
RL   Submitted (26-JUL-1991) by:
RL   Pall M.L., Department of Genetics and Cell Biology,
RL   Washington State University, Pullman, WA 99164-4234, USA.
XX
CC   NCBI gi: 173018
CC   NM (pYADE4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron, ori
FT                   3. yeast, TRP1 gene
FT                   4. yeast, AMP gene
FT                   -> pYADE4 6037bp"
FT   CDS             complement(1895..2755)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3041..3742
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
XX
SQ   Sequence 6037 BP; 1667 A; 1270 C; 1359 G; 1741 T; 0 other;
     accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc
     gggggatcct ctagagtcga gcttttgtgt attacgatat agttaatagt tgatagttga
     ttgtatgctt tttgtagctt gatattctat ttaccaagaa gaaacaagaa gtgataaaaa
     caacaagaga gcagtagtaa gagtatttcg agtgtgaaaa aagtcgctac tggcactcta
     tttatatgtg ataggcatgc tatagcttta ccaaaaagtg aaccccattt ctatgctctc
     ctctgccttt tttttttttt tttttttcat tctctcaatc tgaaattctc ttatttctcc
     aacttataag ttggagatgc ccggtgttcc ggcagaggag atcagtctcg tgaagtggat
     ggtttcccgc ctgcgggcaa aacgtcataa catttttatg agcgaaagcc gttaatgaag
     acaaaatccc ttaattaaaa cattagaatg gtgattagaa aggcaggatt aatcagttac
     acaggctgta accggagaga cggatcataa ggcaattttt agataagact ggttagagtt
     cttggcatca gaaaatttga gaaacgattt ttccgtttgt ttgcccctac gttttgcccc
     tttgatctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     cgaggccctt tcgtcttcaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga
     gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc
     ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg
     cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat
     tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag
     tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg
     taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca
     actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa
     aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca
     ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa
     ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc
     tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     cacttgcctg cagcttctca atgatattcg aatacgcttt gaggagatac agcctaatat
     ccgacaaact gttttacaga tttacgatcg tacttgttac ccatcattga attttgaaca
     tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
     gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
     acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
     tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
     tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
     attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg
     agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat acgttgtcct
     ggccacggca gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag
     gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt
     ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctttgcaaa ttaaagcctt
     cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
     tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac
     tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag
     agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgatatcgac
     aaaggaaaag gggcctgttt actcacaggc ttttttcaag taggtaatta agtcgtttct
     gtctttttcc ttcttcaacc caccaaaggc catcttg
//