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ID   PYADH2     preliminary; circular DNA; SYN; 6445 BP.
XX
AC   L11060;
XX
DT   07-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pYADH2 - incomplete.
XX
KW   cloning vector; promoter; beta-lactamase gene; TRP1 gene;
KW   CYC1 gene; terminator; ADH2; replication origin.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pYADE4 from TRP1 gene & AMP gene
RC   pYADH2 from AMP gene & TRP1 gene & ADH2 gene & CYC1 gene
RC   pYSVE2 from TRP1 gene & AMP gene
RA   Brunelli J.P., Pall M.L.;
RT   "A series of yeast vectors for expression of cDNAs and other DNA
RT   sequences";
RL   Yeast 9:1299-1308(1993).
XX
RN   [2]
RP   1-6445
RC   pYADH2 from AMP gene & TRP1 gene & ADH2 gene & CYC1 gene
RA   Brunelli J.P., Pall M.P.;
RT   "A series of of yeast/E. coli lambda expression vectors designed for
RT   directional cloning of cDNAs and cre/lox mediated plasmid excision";
RL   Yeast 9:1309-1318(1993).
XX
RN   [3]
RP   1-6445
RC   pYADH2
RA   Pall M.P.;
RT   ;
RL   Submitted (07-JUN-1993) by:
RL   Pall M.P., Department of Genetics and Cell Biology,
RL   Washington State University, Pullman, WA 99164-4234, USA.
XX
CC   NCBI gi: 310741
CC   NM (pYADH2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (bacteriophage P1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast, AMP gene
FT                   3. yeast, TRP1 gene
FT                   4. yeast, ADH2 gene promoter
FT                   5. yeast, CYC1 gene terminator
FT                   -> pYADH2 6445bp"
FT   misc_binding    1..80
FT                   /note="MCS"
FT   promoter        81..668
FT                   /note="PRO yeast ADH2 gene"
FT   rep_origin      750..1500
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             1799..2890
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp);
FT                   16 stops when translation attempted, frame 1, code 0"
FT   CDS             2890..3790
FT                   /note="ANT yeast TRP1 gene;
FT                   6 stops when translation attempted, frame 1, code 0"
FT   rep_origin      3790..5465
FT                   /note="ORI yeast 2 micron"
FT   misc_feature    5660..5745
FT                   /note="SIT lox site"
FT   misc_feature    5785..5850
FT                   /note="SIT lox site"
FT   terminator      5880..6420
FT                   /note="TER yeast CYC1 terminator"
XX
SQ   Sequence 6445 BP; 1790 A; 1354 C; 1443 G; 1858 T; 0 other;
     gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
     ccgggggatc ctctagagtc gagcttttgt gtattacgat atagttaata gttgatagtt
     gattgtatgc tttttgtagc ttgatattct atttaccaag aagaaacaag aagtgataaa
     aacaacaaga gagcagtagt aagagtattt cgagtgtgaa aaaagtcgct actggcactc
     tatttatatg tgataggcat gctatagctt taccaaaaag tgaaccccat ttctatgctc
     tcctctgcct tttttttttt tttttttttc attctctcaa tctgaaattc tcttatttct
     ccaacttata agttggagat gcccggtgtt ccggcagagg agatcagtct cgtgaagtgg
     atggtttccc gcctgcgggc aaaacgtcat aacattttta tgagcgaaag ccgttaatga
     agacaaaatc ccttaattaa aacattagaa tggtgattag aaaggcagga ttaatcagtt
     acacaggctg taaccggaga gacggatcat aaggcaattt ttagataaga ctggttagag
     ttcttggcat cagaaaattt gagaaacgat ttttccgttt gtttgcccct acgttttgcc
     cctttgatct ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtcttc aagaattaat tcggtcgaaa aaagaaaagg agagggccaa
     gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca
     gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc
     ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt
     attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca
     agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt
     cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc
     caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt
     aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct
     cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg
     aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta
     gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt
     gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat
     ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg
     tgcacttgcc tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat
     atccgacaaa ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa
     catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat
     atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca
     tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt
     gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
     cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
     gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
     atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
     tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
     tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
     ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata tgcatagtac
     cgagaaacta gtggatctaa acataaaatc tgtaaaataa caagatgtaa agataatgct
     aaatcatttg gctttttgat tgattgtaca ggaaaatata catcgcaggg ggttgacttt
     taccatttca ccgcaatgga atcaaacttg ttgaagagaa tgttcacagg cgcatacgct
     acaatgaccc gattcttgct agccttttct cggtcttgca aacaaccgcc gcggccggtc
     gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag ggttccggat
     cgcggccgct cgacctgcag ccaagctagc ttggctggac gtaaactcct cttcagacct
     aataacttcg tatagcatac attatacgaa gttatattaa gggttattga atatgatcgg
     aattggtcga tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga
     agcacgctta tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga
     aagaaatgag gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa
     gattgaataa ggcgcatttt tcttcaaagc tttgcaaatt aaagccttcg agcgtcccaa
     aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
     aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
     aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
     gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa aggaaaaggg
     gcctgtttac tcacaggctt ttttcaagta ggtaattaag tcgtttctgt ctttttcctt
     cttcaaccca ccaaaggcca tcttg
//