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ID   PYECEN330  preliminary; circular DNA; SYN; 6300 BP.
AC   V01297; J01317; ATCC53232;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pYe(CEN3)30 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pYe(CEN3)30 from pYe[CDC10]1 & YRp7'
RA   ;
RT   ;
RL   Unpublished (1990).
RL   U.S. Patent No. 4,935,350 dated June 19, 1990.
RN   [2]
RC   pYe[CDC10]1 from pLC544 & yeast chromosome III
RC   pYe(CEN3)41 from pYe[CDC10]1
RC   pYe(CEN3)30 from pYe[CDC10]1 & YRp7'
RC   pYe35 from pLC544 & pYe[CDC10]1
RC   pYe6 from pLC544 & YRp7'
RA   Fitzgerald-Hayes M., Clarke L., Carbon J.;
RT   "Nucleotide sequence comparisons and functional analysis of yeast
RT   centromere DNAs";
RL   Cell 29:235-244(1982).
RN   [3]
RC   YRp26 from Yrp series
RC   YCpPHO from pYe(CEN3)41 & PHO5
RC   YCpGAL from pYe(CEN3)41 & GAL10
RA   Eldarov M.A., Karpychev I.V., Kuprina N.Y., Nikolaishvili N.T.,
RA   Larionov V.L., Scriabin K.G.;
RT   "Behavior of artificial mini-chromosomes in yeast during the
RT   induced transcription of centromeric DNA";
RL   Mol. Gen. Mikrobiol. Virusol. 1988:39-43(1987).
CC   Contains unique BamHI, BglII, XbaI, NruI, and SalI sites, with XbaI
CC   and BglII inactivating TRP1. (personal communication)
CC   Constructed by inserting a 627 bp BamHI/Sau3AI fragment containing
CC   CEN3 into the BamHI site of YRp7'. [2]
CC   Demonstrates mitotic stability increased over YRp7'; 60-90% of
CC   transformed S. cerevisiae J17 maintain the Trp+ phenotype after 20
CC   generations. [2]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.3; BglII--6.3; SalI--6.3; EcoRI--4.6, 1.7. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pYe CEN3 30)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli JA228)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pLC544 BamHI
FT                   2. yeast chromosome III poly(dA:dt) connector 8000bp
FT                   -> pYe[CDC10]1
FT                   1. pYe[CDC10]1 HindIII-BamHI 2000bp, CEN3
FT                   Sau3A-BamHI 627bp, CEN3
FT                   2. YRp7' BamHI
FT                   -> pYe(CEN3)30"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SalI-NruI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN3"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;