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ID   PYI13G     preliminary; circular DNA; SYN; 13000 BP.
AC   ATCC37298;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pYI13G - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBRG1 from pBR322 & pAO43
RC   pYR12GR, pYR12GS from pAO43 & YRp12
RC   pYE13G from pAO43 & YEp13, LEU2 gene
RC   pMC3G from pAO43 & pMC1571
RC   pYI13G from pYE13G
RA   Webster T.D., Dickson R.C.;
RT   "Direct selection of Saccharomyces cerevisiae resistant to the
RT   antibiotic G418 following transformation with a DNA vector
RT   carrying the kanamycin-resistance gene of Tn903";
RL   Gene 26:243-252(1983).
CC   This is a direct selection vector constructed by deleting the 2 micron
CC   sequences from YEp13G with SalI.  Transformants with multiple
CC   integrated copies of the plasmid are resistant to G418. [2]
CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pYI13G)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DG75)(Saccharomyces cerevisiae)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pYE13G remove SalI-SalI 5000bp,
FT                   \ yeast ori/tet
FT                   -> pYI13G 13000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT vertebrate G418 resistance gene (G418)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;