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ID   PYR12GR    preliminary; circular DNA; SYN; 8300 BP.
AC   ATCC37275;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pYR12GR - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBRG1 from pBR322 & pAO43
RC   pYR12GR, pYR12GS from pAO43 & YRp12
RC   pYE13G from pAO43 & YEp13, LEU2 gene
RC   pMC3G from pAO43 & pMC1571
RC   pYI13G from pYE13G
RA   Webster T.D., Dickson R.C.;
RT   "Direct selection of Saccharomyces cerevisiae resistant to the
RT   antibiotic G418 following transformation with a DNA vector
RT   carrying the kanamycin-resistance gene of Tn903";
RL   Gene 26:243-252(1983).
CC   pYR12GR was constructed from YRp12 by inserting a HaeII fragment from
CC   pA043 into the PvuII site. [2]
CC   A general purpose replicating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pYR12GR)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DG75)(Saccharomyces cerevisiae)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> R6-5
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pML21, mini-ColE1 segment
FT                   -> pAO100
FT                   1. pAO100
FT                   -> pAO2 3300bp
FT                   1. pACYC177 HaeII-HaeII 1430bp 1596..3026, Tn903 kan
FT                   2. pAO2 3300bp
FT                   -> pAO43 4700bp
FT                   1. pAO43 HaeII-HaeII 1400bp, kan gene/#V00359
FT                   Klenow:Klenow
FT                   2. YRp12 PvuII 6798bp 3059..3059
FT                   -> pYR12GR 8300bp"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT Saccharomyces G418 resistance gene (G418)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;