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ID   PYSVE2     preliminary; circular DNA; SYN; 6010 BP.
XX
AC   M74015;
XX
DT   01-NOV-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pYSVE2 - incomplete, TRP1AMPr.
XX
KW   cloning vector; promoter; beta-lactamase gene; TRP1 gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6010
RC   pYADE4 from TRP1 gene & AMP gene
RC   pYADH2 from AMP gene & TRP1 gene & ADH2 gene & CYC1 gene
RC   pYSVE2 from TRP1 gene & AMP gene
RA   Brunelli J.P., Pall M.L.;
RT   "A series of yeast vectors for expression of cDNAs and other DNA
RT   sequences";
RL   Yeast 9:1299-1308(1993).
XX
RN   [2]
RP   1-6010
RC   pYSVE2
RA   Pall M.L.;
RT   ;
RL   Submitted (26-JUL-1991) by:
RL   Pall M.L., Department of Genetics and Cell Biology,
RL   Washington State University, Pullman, WA 99164-4234, USA.
XX
CC   NCBI gi: 173015
CC   NM (pYSVE2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast, TRP1 gene
FT                   3. yeast, AMP gene
FT                   -> pYSVE2 6010bp"
FT   CDS             complement(1868..2728)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3041..3715
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
XX
SQ   Sequence 6010 BP; 1629 A; 1300 C; 1385 G; 1696 T; 0 other;
     gtaccaggaa tatatttctt tgggttagtc aagtactctg acatgttatt ttcgtcccac
     aacacgtttt tcttgatatt ggcatctgtg tacgaatacc cttcagcttg accagagtgt
     ctgccaaaga taccatgcaa gtttggacca accttatgtg ggccaccctt ttccacggtg
     tggcattgta gacatctagt cttgaaaagt gtagcacctt tcttagcaga accggccttg
     aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagctttttg
     caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     gactttccac accctaactg acacacattc cacagagatc tctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa
     ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
     gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
     aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
     tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
     aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
     aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
     tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga
     ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
     catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
     agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
     agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg
     tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa
     tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat
     tactgagtag tatttattta agtattgttt gtgcacttgc ctgcagcttc tcaatgatat
     tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
     ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
     tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
     ttcctttgat attggatcat atgcatagta ccgagaaact agtgcgaagt agtgatcagg
     tattgctgtt atctgatgag tatacgttgt cctggccacg gcagaagcac gcttatcgct
     ccaatttccc acaacattag tcaactccgt taggcccttc attgaaagaa atgaggtcat
     caaatgtctt ccaatgtgag attttgggcc attttttata gcaaagattg aataaggcgc
     atttttcttc aaagctttgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa
     ggttttcagt ataatgttac atgcgtacac gcgtctgtac agaaaaaaaa gaaaaatttg
     aaatataaat aacgttctta atactaacat aactataaaa aaataaatag ggacctagac
     ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtggggggag ggcgtgaatg
     taagcgtgac ataactaatt acatgatatc gacaaaggaa aaggggcctg tttactcaca
     ggcttttttc aagtaggtaa ttaagtcgtt tctgtctttt tccttcttca acccaccaaa
     ggccatcttg
//