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ID   PZL1       preliminary; circular DNA; SYN; 4307 BP.
AC   IG1149;
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phagemid vector pZL1 - complete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pZL1 from pSPORT1 & phage P1 inc locus & lambda, loxP/incA genes
RA   D'Alessio J.M., Bebee R., Hartley J.L., Noon M.C., Polayes D.;
RT   ;
RL   Focus 14:76-76(1992).
CC   NM (pZL1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   HO (E.coli)
CC   CP (high)
CC   FN (cloning up to 10000 bp)(expression)(transcription)
CC   SE (color blue/white)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSPORT1 BspHI/BspI 4109bp 2562..2562,
FT                   \ may be 3570
FT                   2. phage P1 BspHI/BspI-BspHI/BspI, incA gene
FT                   -> plasmid
FT                   1. plasmid BspHI/BspI
FT                   2. lambda BspHI/BspI 48502bp 40643..40643,
FT                   \ loxP gene
FT                   -> pZL1 4307bp"
FT   CDS             complement(>1..<400)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    191..280
FT                   /note="MCS AatII-NspHI-AflIII-BsaAI-PstI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   CDS             complement(470..591)
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    552..552
FT                   /note="SIT unique NarI"
FT   misc_binding    582..582
FT                   /note="SIT unique Esp3I"
FT   misc_binding    687..687
FT                   /note="SIT unique HpaI"
FT   misc_binding    743..743
FT                   /note="SIT unique EcoRI"
FT   misc_binding    984..984
FT                   /note="SIT unique ApaI"
FT   misc_binding    1009..1009
FT                   /note="SIT unique BstEI"
FT   misc_binding    1177..1177
FT                   /note="SIT unique BclI"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacI gene"
FT   rep_origin      complement(861..861)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2252..2252
FT                   /note="SIT unique AlwNI"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda loxP"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2771..2771
FT                   /note="SIT unique Eam1105I"
FT   misc_binding    2843..2843
FT                   /note="SIT unique BsaI"
FT   misc_binding    3254..3254
FT                   /note="SIT unique ScaI"
FT   misc_binding    3371..3371
FT                   /note="SIT unique XmnI"
FT   CDS             complement(0..0)
FT                   /note="GEN bacteriophage lambda incA gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   misc_binding    4070..4070
FT                   /note="SIT unique DraIII"
FT   misc_binding    4176..4176
FT                   /note="SIT unique NaeI"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;