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ID   SMART2     preliminary; circular DNA; SYN; 9600 BP.
AC   ATCC37588;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Drosophila/E.coli cosmid vector smart2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   LoristX from Lorist2
RC   Loristet from LoristX & pRE3
RC   plasmid from cosPneo & ppi25.7
RC   plasmid2 from plasmid & LoristX
RC   smart2 from plasmid2 & Loristet
RA   Speek M., Raff J.W., Harrison-Lavoie K., Little P.F., Glover D.M.;
RT   "Smart2, a cosmid vector with a phage lambda origin for both
RT   systematic chromosome walking and P-element-mediated gene
RT   transfer in Drosophila";
RL   Gene 64:173-177(1988).
RN   [2]
RC   pPvc1 from ppi25.7 & Carnegie 20 or car20
RC   Pc[ry] from ppi25.7 & linker & pPvc1
RC   ppi25.7wc from ppi25.7 & Pc[ry]
RA   Karess R., Rubin G.M.;
RT   "Analysis of P transposable element functions in Drosophila";
RL   Cell 38:135-146(1984).
CC   Cosmid vector for chromosome walking and P-element-mediated germline
CC   transformation in Drosophila.
CC   G418 resistance (neo) is expressed under the control of the Drosophila
CC   hsp70 promoter. There are no pBR322 sequences in the vector.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--9.6; SalI--9.6. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (smart2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli)(Drosophila melanogaster)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Lorist2 remove SstI-BglI 313bp 573..885,
FT                   \ LoristB 312bp 541..853/5252bp
FT                   T4 DNA polymerase
FT                   XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT                   XhoI-XhoI
FT                   -> LoristX 5252bp
FT                   1. pBR322 4361bp, tet gene
FT                   -> pRE3
FT                   1. LoristX remove NheI-NruI 1340bp 3876..5216,
FT                   \ LoristB 4104..5444/3912bp
FT                   T4 DNA polymerase
FT                   2. pRE3 EcoRI-BsmI 1361bp 4360..4361..1360, tet gene
FT                   -> Loristet 5273bp
FT                   1. pBR322 PvuII 4361bp 2069..2069
FT                   2. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   3. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   -> pcos4EMBL 6300bp [2 tandem cos with PvuII between]
FT                   1. lambda
FT                   2. Drosophila white locus
FT                   -> lambda 6.1
FT                   1. pBR322 4361bp
FT                   2. lambda 6.1, Drosophila white locus
FT                   -> p6.1
FT                   1. p6.1, Drosophila white locus
FT                   2. pUC8, MCS
FT                   3. pcos4EMBL, lambda cos
FT                   4. Carnegie 1, Drosophila P element/3616bp
FT                   -> cosP
FT                   1. Carnegie 4, Drosophila P element/3631bp
FT                   2. hsp70-neo, neo gene
FT                   -> Ca4hsneo
FT                   1. cosP, Drosophila P element
FT                   2. Ca4hsneo, neo gene
FT                   -> cosPneo
FT                   1. cosPneo remove small XbaI-SstI
FT                   2. ppi25.7 XbaI-SstI, 17C right hand P element
FT                   -> cosmid [2 P elements]
FT                   1. cosmid remove small EcoRI-EcoRI
FT                   2. LoristX EcoRI-EcoRI 1862bp,
FT                   \ Sp6/MCS/T7 - LoristB 531..2393
FT                   -> cosmid2
FT                   1. cosmid2 SalI-SalI 6200bp,
FT                   \ Sp6/MCS/T7/P element/hsp neo
FT                   reverse transcriptase fill in:reverse transcriptase
FT                   \ fill in
FT                   2. Loristet large EcoRI-EcoRI 3400bp,
FT                   \ cos/tet/cor/P element/LoristB 2393..5614..531
FT                   reverse transcriptase fill in:reverse transcriptase
FT                   \ fill in
FT                   -> smart2 9600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT Drosophila G418 resistance gene (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;