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ID   SYRINX2A   preliminary; circular DNA; SYN; 46000 BP.
AC   ATCC37543;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda Syrinx 2A - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   EMBL3A from EMBL3
RC   piAN7 from linker & piVX & pBR322
RC   piAN13 from piAN7 & pUC13
RC   piMT5lambdaSX from piAN13 & lambda
RC   lambda R76d2 from EMBL3A & lambda cI857 Sam7 & lambda gtWES.lambdaB
RC   Syrinx 2A from EMBL3A & Charon 4A & lambda R76d2
RA   Lutz C.T., Hollifield W.C., Seed B., Davie J.M., Huang H.V.;
RT   "Syrinx 2A: an improved lambda phage vector designed for screening
RT   DNA libraries by recombination in vivo";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:4379-4383(1987).
CC   Resuspend the freeze-dried lysate in 1 ml SM buffer or 10 mM MgSO4.
CC   Designed for recombinational phage library screening using piAN13
CC   plasmid vector.
CC   Medium is 1592 SM buffer.
CC   NM (Syrinx 2A)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (bacteria-free lysate)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. EMBL3 29237bp
FT                   mutation, to Aam32 Bam1
FT                   -> EMBL3A 29237bp
FT                   1. EMBL3A EcoRI-SstII 5890bp 34499..40389, linker
FT                   2. lambda cI857 Sam7 remove HindII-NruI 868bp,
FT                   \ lambda 40942..41810
FT                   SstII-HindIII 3752bp 40389..44141
FT                   3. lambda gtWES.lambdaB HindIII-right end 4361bp,
FT                   \ lambda 44141..48502
FT                   -> lambda R76d2 14003bp
FT                   1. EMBL3A left end-EcoRI 34499bp 1..34499, left arm
FT                   2. Charon 4A EcoRI-EcoRI 6600bp, lac5
FT                   3. Charon 4A EcoRI-EcoRI 7700bp, bio256
FT                   4. lambda R76d2 EcoRI-right end, right arm
FT                   -> Syrinx 2A 46000bp [lambda Aam32 Bam1 Sam100]"
FT   misc_binding    0..0
FT                   /note="MCS SalI-BamHI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-BamHI-EcoRI-XbaI-SstI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;