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ID U1 preliminary; circular DNA; SYN; 1 BP.
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AC IG6013; S60427; S60428; S60430; S60431;
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DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector U1 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pMC1neo-polyA+, pMC1neo-polyA- from neo gene and pMC1; [Stratagene]
RC pAcneoAn+ from pMC1neo-polyA+ & chicken beta-actin promoter
RC pAcneoAn- from pMC1neo-polyA- & chicken beta-actin promoter
RC pXSP64 from pSP64
RC pXSP-SupF from pCDM8 & pXSP64
RC U2 from pAGS-lacZ & pXSP-SupF & pAcneoAn+
RC U2pA from pAGS-lacZ & pXSP-SupF & pAcneoAn-
RC U1 from U2
RA Niwa H., Araki K., Kimura S., Taniguchi S., Wakasugi S.,
RA Yamamura K.;
RT "An efficient gene-trap method using poly A trap vectors and
RT characterization of gene-trap events";
RL J. Biochem. 113:343-349(1993).
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RN [2]
RC piH3 from piSV & pSV2 & linker & oligo, hCMV immediate-early/HIV LTR
RA Seed B., Aruffo A.;
RT "Molecular cloning of the CD2 antigen, the T-cell erythrocyte
RT receptor, by a rapid immunoselection procedure";
RL Proc. Natl. Acad. Sci. U.S.A. 84:3365-3369(1987).
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RN [3]
RC pRSV-lacZ from pRSVcat & pCH110 or pSV-lacZ
RC pAc-lacZ from pCH110 & chicken beta-actin gene & linker
RC pAG-lacZ from pAc-lacZ & linker & pKCR, rabbit beta-globin gene
RC pAGS-lacZ from pAG-lacZ & pSV2-cat
RC pAcS-lacZ from pAc-lacZ & pSV2-cat
RC pAGS-3 from pAGS-lacZ & pCDM8
RC pAGS3-IL5 from pAGS-3 & IL-5 gene
RA Miyazaki J., Takaki S., Araki K., Tashiro F., Tominaga A.,
RA Takatsu K., Yamamura K.;
RT "Expression vector system based on the chicken beta-actin promoter
RT directs efficient production of interleukin-5";
RL Gene 79:269-277(1989).
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CC exon trap vectors. GenBank sequences are the trapped fragments.
CC also U2 vector.
CC NM (U1)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)(Drosophila)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pMC1neo-polyA+)(pMC1neo-polyA-)(pCDM8)(pXSP64 from pSP64)
CC PA (pAGS-lacZ)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pCH110 AccI 7128bp 6604..6604,SV40 early pro
FT XhoI linker 6bp ctcgag,
FT remove XhoI-HindIII 521bp 6604..7125, 6607bp
FT 2. chicken NcoI, beta-actin gene
FT S1 nuclease
FT HindIII linker 6bp aagctt
FT XhoI-HindIII 1300bp, chicken beta-actin promoter
FT -> pAc-lacZ 7900bp
FT 1. pAc-lacZ remove MboII-HindIII 40bp, actin splice
FT \ 7900bp
FT XbaI linker 6bp tctaga:
FT 2. pKCR ApaI-EcoRI 92bp, rabbit beta-globin splice acc
FT XbaI linker 6bp tctaga:HindIII linker 6bp aagctt
FT -> pAG-lacZ 8000bp
FT 1. pAG-lacZ remove BamHI-EcoRI 450bp or 751bp, SV40
FT \ pCH110 3287..3737 or 3737..4488/7500bp
FT :BamHI linker 6bp ggatcc
FT 2. pSV2-cat SphI-HpaI 1701bp 3505..5003..203, SV40 ori
FT 3. pSV2-cat BamHI-HpaI 135bp 3370..3505, SV40 polyA
FT -> pAGS-lacZ 6000bp
FT 1. pSP64 XbaI 2999bp 29..29
FT fill in
FT -> pXSP64 2999bp
FT 1. pCDM8 PvuII-MnlI 230bp 1171..1401, SupF
FT MnlI CCTCNNNNNN^N
FT :blunt end
FT 2. pXSP64 EcoRI-AatII 2171bp 53..2224
FT blunt end:blunt end
FT -> pXSP-SupF 2400bp
FT 1. lambda
FT 2. E. coli lac operon, lacI/lacPO/Z/Y/A
FT -> lambda h80dlac
FT 1. pMB9 HpaI 5300bp
FT 2. lambda h80dlac HindII-HindIII 2800bp, lacI/lacPO
FT -> pMC1 8100bp
FT 1. pMC1neo-polyA- remove XhoI-MluI 800bp, neo
FT \ 2800bp
FT 2. chicken XhoI-HindIII 1300bp, beta-actin promoter
FT -> pAcneoAn- 4100bp
FT 1. pAGS-lacZ XbaI-BamHI 4000bp, splice/lacZ/SV40 polyA
FT :blunt end
FT 2. pXSP-SupF SmaI-BamHI 2200bp
FT :XhoI linker 6bp ctcgag
FT 3. pAcneoAn- BamHI 4100bp
FT XbaI linker 6bp tctaga
FT XhoI-XbaI 2000bp
FT -> U2 8200bp
FT 1. U2 remove HindIII-KpnI, lacZ start
FT -> U1 8000bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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