back Return to this vector's summary.
ID   U1         preliminary; circular DNA; SYN; 1 BP.
XX
AC   IG6013; S60427; S60428; S60430; S60431;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Drosophila/E.coli plasmid vector U1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMC1neo-polyA+, pMC1neo-polyA- from neo gene and pMC1; [Stratagene]
RC   pAcneoAn+ from pMC1neo-polyA+ & chicken beta-actin promoter
RC   pAcneoAn- from pMC1neo-polyA- & chicken beta-actin promoter
RC   pXSP64 from pSP64
RC   pXSP-SupF from pCDM8 & pXSP64
RC   U2 from pAGS-lacZ & pXSP-SupF & pAcneoAn+
RC   U2pA from pAGS-lacZ & pXSP-SupF & pAcneoAn-
RC   U1 from U2
RA   Niwa H., Araki K., Kimura S., Taniguchi S., Wakasugi S.,
RA   Yamamura K.;
RT   "An efficient gene-trap method using poly A trap vectors and
RT   characterization of gene-trap events";
RL   J. Biochem. 113:343-349(1993).
XX
RN   [2]
RC   piH3 from piSV & pSV2 & linker & oligo, hCMV immediate-early/HIV LTR
RA   Seed B., Aruffo A.;
RT   "Molecular cloning of the CD2 antigen, the T-cell erythrocyte
RT   receptor, by a rapid immunoselection procedure";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:3365-3369(1987).
XX
RN   [3]
RC   pRSV-lacZ from pRSVcat & pCH110 or pSV-lacZ
RC   pAc-lacZ from pCH110 & chicken beta-actin gene & linker
RC   pAG-lacZ from pAc-lacZ & linker & pKCR, rabbit beta-globin gene
RC   pAGS-lacZ from pAG-lacZ & pSV2-cat
RC   pAcS-lacZ from pAc-lacZ & pSV2-cat
RC   pAGS-3 from pAGS-lacZ & pCDM8
RC   pAGS3-IL5 from pAGS-3 & IL-5 gene
RA   Miyazaki J., Takaki S., Araki K., Tashiro F., Tominaga A.,
RA   Takatsu K., Yamamura K.;
RT   "Expression vector system based on the chicken beta-actin promoter
RT   directs efficient production of interleukin-5";
RL   Gene 79:269-277(1989).
XX
CC   exon trap vectors. GenBank sequences are the trapped fragments.
CC   also U2 vector.
CC   NM (U1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Drosophila)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMC1neo-polyA+)(pMC1neo-polyA-)(pCDM8)(pXSP64 from pSP64)
CC   PA (pAGS-lacZ)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCH110 AccI 7128bp 6604..6604,SV40 early pro
FT                   XhoI linker 6bp ctcgag,
FT                   remove XhoI-HindIII 521bp 6604..7125, 6607bp
FT                   2. chicken NcoI, beta-actin gene
FT                   S1 nuclease
FT                   HindIII linker 6bp aagctt
FT                   XhoI-HindIII 1300bp, chicken beta-actin promoter
FT                   -> pAc-lacZ 7900bp
FT                   1. pAc-lacZ remove MboII-HindIII 40bp, actin splice
FT                   \ 7900bp
FT                   XbaI linker 6bp tctaga:
FT                   2. pKCR ApaI-EcoRI 92bp, rabbit beta-globin splice acc
FT                   XbaI linker 6bp tctaga:HindIII linker 6bp aagctt
FT                   -> pAG-lacZ 8000bp
FT                   1. pAG-lacZ remove BamHI-EcoRI 450bp or 751bp, SV40
FT                   \ pCH110 3287..3737 or 3737..4488/7500bp
FT                   :BamHI linker 6bp ggatcc
FT                   2. pSV2-cat SphI-HpaI 1701bp 3505..5003..203, SV40 ori
FT                   3. pSV2-cat BamHI-HpaI 135bp 3370..3505, SV40 polyA
FT                   -> pAGS-lacZ 6000bp
FT                   1. pSP64 XbaI 2999bp 29..29
FT                   fill in
FT                   -> pXSP64 2999bp
FT                   1. pCDM8 PvuII-MnlI 230bp 1171..1401, SupF
FT                   MnlI CCTCNNNNNN^N
FT                   :blunt end
FT                   2. pXSP64 EcoRI-AatII 2171bp 53..2224
FT                   blunt end:blunt end
FT                   -> pXSP-SupF 2400bp
FT                   1. lambda
FT                   2. E. coli lac operon, lacI/lacPO/Z/Y/A
FT                   -> lambda h80dlac
FT                   1. pMB9 HpaI 5300bp
FT                   2. lambda h80dlac HindII-HindIII 2800bp, lacI/lacPO
FT                   -> pMC1 8100bp
FT                   1. pMC1neo-polyA- remove XhoI-MluI 800bp, neo
FT                   \ 2800bp
FT                   2. chicken XhoI-HindIII 1300bp, beta-actin promoter
FT                   -> pAcneoAn- 4100bp
FT                   1. pAGS-lacZ XbaI-BamHI 4000bp, splice/lacZ/SV40 polyA
FT                   :blunt end
FT                   2. pXSP-SupF SmaI-BamHI 2200bp
FT                   :XhoI linker 6bp ctcgag
FT                   3. pAcneoAn- BamHI 4100bp
FT                   XbaI linker 6bp tctaga
FT                   XhoI-XbaI 2000bp
FT                   -> U2 8200bp
FT                   1. U2 remove HindIII-KpnI, lacZ start
FT                   -> U1 8000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//