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ID   YCP19      preliminary; circular DNA; SYN; 10600 BP.
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AC   M13000; ATCC37364;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YCp19 or pNN130 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp16, YRp17 from YRp12
RC   YCp17 or pNN130 from YRp17 & yeast Sc4137, CEN4
RC   [pNN279, pNN280, pNN281, pNN282, pNN283 from pNN series]
RC   [pNN284, pNN285 from pNN series]
RA   Stinchcomb D.T., Mann C., Davis R.W.;
RT   "Centromeric DNA from Saccharomyces cerevisiae";
RL   J. Mol. Biol. 158:157-179(1982).
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RN   [2]
RC   YRp12 or YIp5-Sc4101 from YIp5 & lambda gt-Sc4101, TRP1 gene
RC   pBR322-Sc2903 from pBR322 & YIp1
RC   YIp5-Sc2903 from YIp5 & pBR322-Sc2903
RC   YIp5-Sc2911 from YIp5-Sc2903 & galactose-inducible RNA
RA   Scherer S., Davis R.W.;
RT   "Replacement of chromosome segments with altered DNA sequences
RT   constructed in vitro";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:4951-4955(1979).
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CC   YCp19 was constructed from YRp17 (ATCC 37078) by inserting a 3.6 kb
CC   BamHI-EcoRI fragment from Sc4137 containing CEN4.  This is a
CC   mitotically stable derivative. [2]
CC   A general purpose Saccharomyces cerevisiae/E.coli shuttle vector
CC   containing centromeric DNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YCp19)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. yeast, CEN4/#M1300/2095bp
FT                   -> lambda Sc4130
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. lambda Sc4130 EcoRI-EcoRI, CEN4/#M1300/2095bp
FT                   -> yeast Sc4137 6456bp
FT                   1. YRp17 BamHI 7002bp 1840..1840
FT                   2. yeast Sc4137 BamHI-EcoRI 3600bp, CEN4
FT                   \pBR322 4360..4361..-..376
FT                   -> YCp19 10600bp [part of pYAC2]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 2095 BP; 681 A; 388 C; 344 G; 682 T; 0 other;
     ctcatgcaat tgctcaaagc gtaaaaattt cacttttcga agagcttgtg gataatacta
     ttgaagatac tcaggatata ccacaagaaa ttgcatacag cggtaaagtt tctatgagca
     aagaagatat aatgaaaagt ataggggagc tattcatttt gaggataaat atcaatctac
     atggatcagt tttagactct ccagaaatta tgtggtcaga accgcagtta gaacccatat
     accaagcaac aagaggttac ttagagatca atcagcgcgt ttcactttta aatcaaagac
     tcgaggtcat ttcagatctc tcaaatgttg aaggaacagc tgggccattc tcatgaagaa
     tatcttgaat ttattgtcat attactagtt ggtgtggaag tccatatatc ggtgatcaat
     atagtggttg acatgctggc tagtcaacat tgagcctttt gatcatgcaa atatattacg
     gtattttaca atcaaatatc aaacttaact attgacttta taacttattt aggtggtaac
     attcttataa aaaagaaaaa aattactgca aaacagtact agcttttaac ttgtatccta
     ggttatctat gctgtctcac catagagaat attacctatt tcagaatgta tgtccatgat
     tcgccgggta aatacatata atacacaaat ctggcttaat aaagtctata atatatctca
     taaagaagtg ctaaattggc tagtgctata tatttttaag aaaatttctt ttgactaagt
     ccatatcgac tttgtaaaag ttcactttag catacatata ttacacgagc cagaaattgt
     aacttttgcc taaaatcaca aattgcaaaa tttaattgct tgcaaaaggt cacatgctta
     taatcaactt ttttaaaaat ttaaaatact tttttatttt ttatttttaa acataaatga
     aataatttat ttattgttta tgattaccga aacataaaac ctgctcaaga aaaagaaact
     gttttgtcct tggaaaaaaa gcactaccta ggagcggcca aaatgccgag gctttcatag
     cttaaactct ttacagaaaa taggcattat agatcagttc gagttttctt attcttcctt
     ccggttttat cgtcacagtt ttacagtaaa taagtatcac ctcttagagt taactatgag
     ataagcaagt atcatctcat ttcatttacc tgaagtcgag taaacagaaa atccaattgt
     tgatgaacct caatgactta gaactatcta tcggcagatc atataaagag gatttaggta
     cctagaggac tgtacctgga gtatatatat atatatatat atattatctc aactatagtc
     catagaggtt tctttcttga ggccttaaac tgctaaagaa tgatattggt ggaatgcaag
     caccaatctc tcttctttcg taactgttca tatacttcaa accaagaatg taacgggcat
     tgacccatcc aaaaccttca gtagctgccc ctttaaagtc agcaccttga ttaccgtatt
     ctgcttcaac acgatgagga tctgttcctc ttgtgacatc atatttttca accacaatac
     cattataatc gacaaaagcc tttgtcatca tgaaaagcca tctataagct agcctattcg
     ttacagttaa ataaccataa gaacggaggc cttcccaagc aagaatttga tggggtgccc
     aaccaaatgg atagtcccat tgtctaattg gtctcgaaat agaaattggg cctcgagaac
     gctccgtaca tgcagctaaa cctccaagca tctctaactt gggtagtgct ttctccacca
     ttttctgtgc ttgctccttc gtggcaagtc cagcccataa tgcccagaat gtagttgcgg
     attcgtatga cgttctgtgc ttgatttttg tgttgtagtc aaagaaaaac cccgactcgt
     catcccacat atatttggta atcttttctt gtctgatttt ggccatttct ttccacatag
     ctgaagttgt tatagaatgg tctaaaggat cttcatattt gtcgttattg aattc