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ID   YCP631     preliminary; circular DNA; SYN; 12260 BP.
AC   ATCC37470;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YCp631 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
CC   A general purpose Saccharomyces cerevisiae/E.coli shuttle vector
CC   containing centromeric DNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YCp631)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YEp24 7769bp, ori
FT                   2. yeast, LYS2 gene
FT                   -> pDA6200
FT                   1. pBR322, ori/amp
FT                   2. pDA6200, LYS gene
FT                   -> YIp600
FT                   1. pLC544
FT                   2. yeast, chromosome III CDC10 gene
FT                   -> pYe(CDC10)1
FT                   1. pLC544
FT                   2. pYe(CDC10)1
FT                   -> pYe(CEN3)11
FT                   1. pBR322, ori/amp
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   3. yeast, ARS1
FT                   4. pYe(CEN3)11, CEN3
FT                   -> pYE41(CEN3)10
FT                   1. YIp600 PvuII
FT                   2. pYE41(CEN3)10 BamHI-EcoRI, CEN3/ARS1
FT                   Klenow:Klenow
FT                   -> YCp631 12260bp"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN3"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;