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ID   YCP631     preliminary; circular DNA; SYN; 12260 BP.
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AC   ATCC37470;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YCp631 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
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CC   A general purpose Saccharomyces cerevisiae/E.coli shuttle vector
CC   containing centromeric DNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YCp631)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 7769bp, ori
FT                   2. yeast, LYS2 gene
FT                   -> pDA6200
FT                   1. pBR322, ori/amp
FT                   2. pDA6200, LYS gene
FT                   -> YIp600
FT                   1. pLC544
FT                   2. yeast, chromosome III CDC10 gene
FT                   -> pYe(CDC10)1
FT                   1. pLC544
FT                   2. pYe(CDC10)1
FT                   -> pYe(CEN3)11
FT                   1. pBR322, ori/amp
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   3. yeast, ARS1
FT                   4. pYe(CEN3)11, CEN3
FT                   -> pYE41(CEN3)10
FT                   1. YIp600 PvuII
FT                   2. pYE41(CEN3)10 BamHI-EcoRI, CEN3/ARS1
FT                   Klenow:Klenow
FT                   -> YCp631 12260bp"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN3"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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