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ID   YCP8PHOCAT preliminary; circular DNA; SYN; 9260 BP.
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AC   ATCC37634;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YCp8phocat - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp7-32cat from YRp7 & pBR325
RC   YIp5-32cat from YRp7-32cat & YIp5
RC   YCp7-32cat from YRp7-32cat & pLA417
RC   [M13mp8::PHO5 from M13mp8 & PHO5]
RC   YRp7-32phocat from YRp7-32cat & M13mp8::PHO5
RC   YIp5-32phocat from YRp7-32phocat & pLA417
RC   YCp7-32phocat from YRp7-32phocat & pLA417
RC   YRp7-17camp from YRp7 & pBR325
RC   YRp8 from YRp7 & YIp5
RC   [M13mp10::CEN6 from M13mp10 & CEN6]
RC   YIp5CEN from YIp5 & M13mp10::CEN6
RC   YCp8 from YRp7 & YIp5CEN
RC   YRp8cat from YRp8 & YRp7-32cat
RC   YRp8phocat from YRp8 & YRp7-32phocat
RC   YCp8cat from YCp8 & YRp7-32cat
RC   YCp8phocat from YCp8 & YRp7-32phocat
RA   Mannhaupt G., Pilz U., Feldmann H.;
RT   "A series of shuttle vectors using chloramphenicol
RT   acetyltransferase as a reporter enzyme in yeast";
RL   Gene 67:287-294(1988).
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CC   One of a series (ATCC 37624 through 37634) of Escherichia
CC   coli/Saccharomyces cerevisiae shuttle vectors for investigating
CC   control of expression in yeast.
CC   A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was
CC   ligated to a 1975 bp BamHI/HindIII fragment of YRp7-32phocat
CC   (ATCC 37625).
CC   The depositor recommends using YRp7-17camp (ATCC 37630) as the
CC   reference for CAT expression and using the CAT assay methodology of
CC   Neumann, et al.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI/HindIII--7.2, 2.1; BglII--6.0, 2.6; EcoRI--7.2,1.5,0.6. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YCp8phocat)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp10 7244bp, ori
FT                   2. yeast HindIII-HindIII 1520bp, CEN6/#X01855/800bp
FT                   -> M13mp10::CEN6
FT                   1. YIp5 5541bp
FT                   2. M13mp10::CEN6, CEN6/#X01855/800bp
FT                   -> YIp5CEN
FT                   1. YRp7 5738bp
FT                   2. YIp5CEN
FT                   -> YCp8
FT                   1. YCp8 BamHI-HindIII 7285bp, YRp7 306..-..5127
FT                   2. YRp7-32phocat HindIII-BamHI 1975bp, cat
FT                   \ YRp7 5127..5738..306..-
FT                   -> YCp8phocat 9260bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN6"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   promoter        0..0
FT                   /note="PRO yeast PHO5 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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