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ID   YCPIF12    preliminary; circular DNA; SYN; 5200 BP.
AC   ATCC87089;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid YCpIF12 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   YCpIF12 from pBR322 & ARS1 & TRP1
RA   Foreman P.K., Davis R.W.;
RT   "Cloning vectors for the synthesis of epitope-tagged, truncated
RT   and chimeric proteins in Saccharomyces cerevisiae";
RL   Gene 144:63-68(1994).
CC   Restriction digests of the clone give the following sizes (kb):
CC   XbaI--3.6, 1.8; BamHI--5.4; EcoRI--5.4. (ATCC staff)
CC   One of several S. cerevisiae shuttle expression vectors (ATCC
CC   (87078-87092) differing in selection marker, reading frame and
CC   orientation of the promoter. Vectors YCpIF10, YCpIF11 and YCpIF12
CC   (ATCC 87087-87089) differ only in the reading frame of the multiple
CC   cloning site. [1]
CC   Care should be taken when cloning into this vector because several
CC   stop codons occur in this reading frame. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Davis R.W., Foreman P.K.
CC   NM (YCpIF12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli HB101)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(shuttle)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1
FT                   -> YCpIF12 5200bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast CEN4"
FT   promoter        0..0
FT                   /note="PRO yeast GAL1"
FT   misc_binding    0..0
FT                   /note="MCS SacI-NotI-XbaI-SpeI-BamHI-SmaI-PstI
FT                   EcoRI-EcoRV-HindIII-ClaI-SalI"
FT   misc_binding    0..0
FT                   /note="MCS unique SacI-NotI-SpeI-BamHI-SmaI-
FT                   EcoRI-ClaI-SalI"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;