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ID   YCPIF15    preliminary; circular DNA; SYN; 5400 BP.
AC   ATCC87090;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid YCpIF15 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   YCpIF15 from pBR322 & ARS1 & TRP1
RA   Foreman P.K., Davis R.W.;
RT   "Cloning vectors for the synthesis of epitope-tagged, truncated
RT   and chimeric proteins in Saccharomyces cerevisiae";
RL   Gene 144:63-68(1994).
CC   Restriction digests of the clone give the following sizes (kb):
CC   XbaI--3.6, 1.8; BamHI--5.4; EcoRI--5.4. (ATCC staff)
CC   Yeast expression vector allowing production of a protein tagged at
CC   the N-terminal end with a hemaglutinin epitope. The encoded nine aa of
CC   the influenza virus hemaglutinin epitope are recognized by the
CC   monoclonal antibody 12CA5,allowing detection and affinity purification
CC   of the recombinant protein. Vectors YCpIF15, YCpIF16 and YCpIF17 (ATCC
CC   87090-87092) differ only in the reading frame of the multiple cloning
CC   site. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Davis R.W., Foreman P.K.
CC   NM (YCpIF15)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli HB101)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(shuttle)(expression)(epitope tag for protein isolation)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1
FT                   -> YCpIF15 5400bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast CEN4"
FT   promoter        0..0
FT                   /note="PRO yeast GAL1"
FT   misc_feature    0..0
FT                   /note="hemaglutinin, influenza virus epitope"
FT   misc_binding    0..0
FT                   /note="MCS SacI-NotI-XbaI-SpeI-BamHI-SmaI-PstI
FT                   EcoRI-EcoRV-HindIII-ClaI"
FT   misc_binding    0..0
FT                   /note="MCS unique SacI-NotI-SpeI-BamHI-SmaI-
FT                   EcoRI-ClaI"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;