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ID   YEP21      preliminary; circular DNA; SYN; 8800 BP.
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AC   ATCC37058;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YEp21 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   from pBR322 & yeast 2 micron
RA   Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S.,
RA   Stinchcomb D.T., Struhl K., Davis R.W.;
RT   "Sterile host yeasts (SHY): a eukaryotic system of biological
RT   containment for recombinant DNA experiments";
RL   Gene 8:17-24(1979).
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CC   A general purpose episomal Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp21)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12 r-m+)(Saccharomyces cerevisiae)(E.coli MM294)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron 2200bp, ori
FT                   3. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> YEp21 8800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//