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ID   YEP432     preliminary; circular DNA; SYN; 6400 BP.
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AC   ATCC77117;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YEp432 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRB315 from pPL7 & TRP1
RC   pRB328 from pPL7 & HIS3
RC   pRB506 from pPL7 & LYS2
RC   YCp401 from YCp50 & pRB506
RC   YCp402 from YCp50 & YEp21
RC   YCp403, YCp404 from YCp50 & YEp6
RC   YCp405 from YCp401 & pBR322
RC   YCp406 from YCp50 & pPL7
RC   YCp407, YCp408 from YCp50 & pRB328
RC   YCp409 from YCp402 & pPL7
RC   YCp410, YCp411 from YCp50 & pRB315
RC   YEp420 or beta72 from YIp5 & yeast 2 micron form B
RC   YEp421, YEp422 from YEp420 & YEp6
RC   YEp423, YEp424 from YEp420 & YEp21
RC   YEp425 from YEp420 & pRB506
RC   YEp427, YEp428 from YEp420 & YRp7
RC   YEp429 from YEp420 & pPL7
RC   YEp430, YEp431 from YEp420 & pRB328
RC   YEp432, YEp433 from YEp420 & pRB315
RC   YEp434, YEp435 from pGM65 & YEp21
RC   YEp436 from pSI4 & YIp5
RC   YEp437 from pSI4 & YEp6
RC   YEp441 from YRp7 & YEp6
RC   YEp442 from YRp7 & YEp21
RA   Ma H., Kunes S., Schatz P.J., Botstein D.;
RT   "Plasmid construction by homologous recombination in yeast";
RL   Gene 58:201-216(1987).
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CC   YE-type shuttle vector. (ATCC staff)
CC   The order of the major features in this plasmid is: EcoRI site - TRP1
CC   - BamHI site - SalI site - ori(2 micron) - ampR.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI/BamHI--5.6, 0.9; EcoRI/SalI--5.4, 1.1; XbaI--3.3, 3.0. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp432)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. yeast EcoRI-EcoRI 4900bp, LYS2 gene
FT                   -> pRB506 9300bp
FT                   1. YIp5 5541bp
FT                   2. yeast 2 micron form B 1300bp, ori
FT                   -> YEp420 6800bp
FT                   1. pBR322 remove 250bp
FT                   linker
FT                   -> pPL7 4100bp
FT                   1. pPL7 remove EcoRI-BamHI 377bp 4360..4361..376,
FT                   \ MCS/3730bp
FT                   2. yeast EcoRI-BamHI 1200bp, TRP1 gene
FT                   -> pRB315 4900bp
FT                   1. YEp420 remove small EcoRI-BamHI 377bp, YIp5 2..379
FT                   \ 6500bp
FT                   recombination
FT                   2. pRB315 PvuI-PvuII 1200bp, TRP1 gene
FT                   -> YEp432 6400bp"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII-BamHI-BsmI-BspMII-EcoRI-
FT                   NruI-SalI-SphI-StyI-Tth111I-XmaIII(EagI)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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