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ID   YEP620     preliminary; circular DNA; SYN; 11700 BP.
AC   ATCC37469;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YEp620 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
CC   Can select LYS+ in yeast at high frequency (>1000/ug).
CC   A general purpose episomal Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp620)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YIp600 EcoRI 9460bp 0..0, 5' to LYS2 gene
FT                   \ pBR322 4360..4360
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2, D fragment/ori
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> YEp620 11695bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI-XbaI-XhoI-SalI-SnaBI-FspII-
FT                   EspI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;