Return to this vector's summary.
ID YG100 preliminary; circular DNA; SYN; 11211 BP.
XX
AC IG0129; M17582; M17583; M17586;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector YG100 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pB8 from pBR322 & Drosophila hsp70 gene
RC lambda 100, lambda 101 from Charon 28 & S.cerevisiae S288C DNA
RC YG100 from lambda 100 & pBR322
RC pYG100BH from pYG100
RC YG101 from lambda 101 & pBR322
RA Ingolia T.D., Slater M.R., Craig E.A.;
RT "Saccharomyces cerevisiae contains a complex multigene family
RT related to the major heat shock-inducible gene of Drosophila";
RL Mol. Cell. Biol. 2:1388-1398(1982).
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RN [2]
RC pHS4, pHS12 from pBR322 & Drosophila hsp70 gene
RC pG3, pG5 from pBR322 & Drosophila hsp70 gene
RC pB8, pBH1, pBH15 from pBR322 & Drosophila hsp70 gene
RA Craig E.A., McCarthy B.J., Wadsworth S.;
RT "Sequence organization of two recombinant plasmids containing
RT genes for the major heat shock-induced protein of D. melanogaster";
RL Cell 16:575-588(1979).
XX
CC NM (YG100)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(yg100)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda 100
FT 2. pBR322
FT -> YG100"
FT misc_feature 0..0
FT /note="HindIII-BamHI fragment of Drosophila yg100
FT replaces HindIII-BamHI fragment in pBR322"
FT CDS 0..0
FT /note="GEN Drosophila hsp70 gene; homology"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(10213..11073)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 11211 BP; 946 A; 1126 C; 1073 G; 968 T; 7098 other;
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nnnnnnnnnn nnnnnnnnnn nnggatcctc tacgccggac gcatcgtggc cggcatcacc
ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
tctctcgttt catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca
tcaagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//