back Return to this vector's summary.
ID   YG100      preliminary; circular DNA; SYN; 11211 BP.
XX
AC   IG0129; M17582; M17583; M17586;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector YG100 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pB8 from pBR322 & Drosophila hsp70 gene
RC   lambda 100, lambda 101 from Charon 28 & S.cerevisiae S288C DNA
RC   YG100 from lambda 100 & pBR322
RC   pYG100BH from pYG100
RC   YG101 from lambda 101 & pBR322
RA   Ingolia T.D., Slater M.R., Craig E.A.;
RT   "Saccharomyces cerevisiae contains a complex multigene family
RT   related to the major heat shock-inducible gene of Drosophila";
RL   Mol. Cell. Biol. 2:1388-1398(1982).
XX
RN   [2]
RC   pHS4, pHS12 from pBR322 & Drosophila hsp70 gene
RC   pG3, pG5 from pBR322 & Drosophila hsp70 gene
RC   pB8, pBH1, pBH15 from pBR322 & Drosophila hsp70 gene
RA   Craig E.A., McCarthy B.J., Wadsworth S.;
RT   "Sequence organization of two recombinant plasmids containing
RT   genes for the major heat shock-induced protein of D. melanogaster";
RL   Cell 16:575-588(1979).
XX
CC   NM (YG100)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(yg100)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 100
FT                   2. pBR322
FT                   -> YG100"
FT   misc_feature    0..0
FT                   /note="HindIII-BamHI fragment of Drosophila yg100
FT                   replaces HindIII-BamHI fragment in pBR322"
FT   CDS             0..0
FT                   /note="GEN Drosophila hsp70 gene; homology"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(10213..11073)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 11211 BP; 946 A; 1126 C; 1073 G; 968 T; 7098 other;
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     ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
     gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
     gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
     cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
     tctctcgttt catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca
     tcaagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//