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ID   YIP28      preliminary; circular DNA; SYN; 7800 BP.
AC   ATCC37055;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YIp28 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   from pBR322 & yeast 2 micron
RA   Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S.,
RA   Stinchcomb D.T., Struhl K., Davis R.W.;
RT   "Sterile host yeasts (SHY): a eukaryotic system of biological
RT   containment for recombinant DNA experiments";
RL   Gene 8:17-24(1979).
CC   A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI
CC   fragment containing LEU2 were cloned into the HindIII and SalI sites,
CC   respectively, of pBR322. Unique restriction sites are in the 3'
CC   untranslated regions of URA3(SmaI) and LEU2(SalI). [2]
CC   Orientations are such that transcription from URA3 is clockwise and
CC   from LEU2 is counterclockwise. [2]
CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp28)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli MM294)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII 4361bp 30..30
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> plasmid 5527bp
FT                   1. ColE1 6646bp, ori
FT                   2. yeast SalI-XhoI 2200bp, LEU2 gene
FT                   3. Ty1-17 gene
FT                   -> pYe(Leu2)10
FT                   1. plasmid SalI 5461bp 1752..1752, pBR322 652
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> YIp28 7661bp [SmaI in URA3, SalI in LEU2, 3' UTR]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;