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ID   YIP33      preliminary; circular DNA; SYN; 6700 BP.
AC   ATCC37064;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YIp33 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   from pBR322 & yeast 2 micron
RA   Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S.,
RA   Stinchcomb D.T., Struhl K., Davis R.W.;
RT   "Sterile host yeasts (SHY): a eukaryotic system of biological
RT   containment for recombinant DNA experiments";
RL   Gene 8:17-24(1979).
CC   This was constructed by inserting a 2.2 kb SalI/XhoI fragment
CC   containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322.
CC   The 5'  - 3' orientation of LEU2 is counterclockwise.  LEU2,
CC   originating from strain X2180-1A, includes a region of Ty1-17. [2]
CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp33)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli MM294)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 SalI 4361bp 652..652
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> YIp33 6561bp [unique SalI in LEU2, 3' UTR; Tyl-17]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;