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ID   YIP532CAT  preliminary; circular DNA; SYN; 6610 BP.
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AC   ATCC37628;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp5-32cat - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp7-32cat from YRp7 & pBR325
RC   YIp5-32cat from YRp7-32cat & YIp5
RC   YCp7-32cat from YRp7-32cat & pLA417
RC   [M13mp8::PHO5 from M13mp8 & PHO5]
RC   YRp7-32phocat from YRp7-32cat & M13mp8::PHO5
RC   YIp5-32phocat from YRp7-32phocat & pLA417
RC   YCp7-32phocat from YRp7-32phocat & pLA417
RC   YRp7-17camp from YRp7 & pBR325
RC   YRp8 from YRp7 & YIp5
RC   [M13mp10::CEN6 from M13mp10 & CEN6]
RC   YIp5CEN from YIp5 & M13mp10::CEN6
RC   YCp8 from YRp7 & YIp5CEN
RC   YRp8cat from YRp8 & YRp7-32cat
RC   YRp8phocat from YRp8 & YRp7-32phocat
RC   YCp8cat from YCp8 & YRp7-32cat
RC   YCp8phocat from YCp8 & YRp7-32phocat
RA   Mannhaupt G., Pilz U., Feldmann H.;
RT   "A series of shuttle vectors using chloramphenicol
RT   acetyltransferase as a reporter enzyme in yeast";
RL   Gene 67:287-294(1988).
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CC   The depositor recommends using YRp7-17camp (ATCC 37630) as the
CC   reference for CAT expression and using the CAT assay methodology of
CC   Neumann, et al.
CC   One of a series (ATCC 37624 through 37634) of Escherichia
CC   coli/Saccharomyces cerevisiae shuttle vectors for investigating
CC   control of expression in yeast.
CC   A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp
CC   HindIII/BamHI fragment of YRp7-32cat (ATCC 37624).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp5-32cat)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp7-32cat HindIII-BamHI 1415bp, cat
FT                   \ YRp7 5127..5738..306..-
FT                   2. YIp5 BamHI-HindIII 5195bp 379..5541..33
FT                   -> YIp5-32cat 6610bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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