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ID   YIP532PCAT preliminary; circular DNA; SYN; 6910 BP.
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AC   ATCC37629;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp5-32phocat - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp7-32cat from YRp7 & pBR325
RC   YIp5-32cat from YRp7-32cat & YIp5
RC   YCp7-32cat from YRp7-32cat & pLA417
RC   [M13mp8::PHO5 from M13mp8 & PHO5]
RC   YRp7-32phocat from YRp7-32cat & M13mp8::PHO5
RC   YIp5-32phocat from YRp7-32phocat & pLA417
RC   YCp7-32phocat from YRp7-32phocat & pLA417
RC   YRp7-17camp from YRp7 & pBR325
RC   YRp8 from YRp7 & YIp5
RC   [M13mp10::CEN6 from M13mp10 & CEN6]
RC   YIp5CEN from YIp5 & M13mp10::CEN6
RC   YCp8 from YRp7 & YIp5CEN
RC   YRp8cat from YRp8 & YRp7-32cat
RC   YRp8phocat from YRp8 & YRp7-32phocat
RC   YCp8cat from YCp8 & YRp7-32cat
RC   YCp8phocat from YCp8 & YRp7-32phocat
RA   Mannhaupt G., Pilz U., Feldmann H.;
RT   "A series of shuttle vectors using chloramphenicol
RT   acetyltransferase as a reporter enzyme in yeast";
RL   Gene 67:287-294(1988).
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CC   One of a series (ATCC 37624 through 37634) of Escherichia
CC   coli/Saccharomyces cerevisiae shuttle vectors for investigating
CC   control of expression in yeast.
CC   A 4920 bp SalI/HindIII fragment of YIp5 was ligated to a 1985 bp
CC   HindIII/SalI fragment of YRp7-32phocat (ATCC 37625).
CC   The depositor recommends using YRp7-17camp (ATCC 37630) as the
CC   reference for CAT expression and using the CAT assay methodology of
CC   Neumann, et al.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--6.6, 0.64; PstI--3.85, 3.3; PvuII--3.7, 3.4. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp5-32phocat)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp7-32phocat HindIII-SalI 1985bp, cat/PHO5
FT                   \ YRp7 5127..5738..582..-
FT                   2. YIp5 SalI-HindIII 4920bp 655..5541..33
FT                   NdeI linker 6bp catatg:NdeI linker 6bp catatg
FT                   NdeI-NdeI 1536bp 39..1575, CEN6
FT                   -> YIp5-32phocat 6905bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   promoter        0..0
FT                   /note="PRO E. coli PHO5 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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