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ID   YIP5KANR   preliminary; circular DNA; SYN; 7100 BP.
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AC   X70480; X70483; X70267; X70268; X70269; X70271; X70272; ATCC37473;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/vertebrate/E.coli plasmid vector YIp5-kanR - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YIp5-kanR from YIp5 & pNG35
RA   Rao T.R., Reddy C.A.;
RT   "A YIp5-kanr plasmid useful for isolating ars from yeast and other
RT   eukaryotes based on G418 resistance selection";
RL   Nucleic Acids Res. 14:7504-7504(1986).
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RN   [2]
RC   pNG16 from pBR322
RC   pNG18 from pNG16 & pML21, Tn903 kan gene
RC   pNG20 from pNG16 & pML21, Tn903 kan gene
RC   pNG23 from pNG18
RC   pNG24 from pNG18
RC   pNG35 from pNG18
RC   pNG39, pNG40, pNG42, pNG43, pNG47, pNG48, pNG49 from pNG35
RA   Grindley N.D., Joyce C.M.;
RT   "Genetic and DNA sequence analysis of the kanamycin resistance
RT   transposon Tn903";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980).
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CC   Vector provides selection markers for transformation of Saccharomyces
CC   cerevisiae or other eukaryotes based on G418 resistance.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp5-kanR)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli C600)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove 844bp 1230..2074,
FT                   \ removes PvuII/3517bp
FT                   -> pNG16 3517bp [amp/tet/ori]
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pNG16 remove EcoRI-SalI 653bp 4360..4361..652,
FT                   \ between amp/tet/2864bp
FT                   2. pML21 EcoRI-SalI 5000bp 9262..14262, Tn903 kan gene
FT                   \ #V00359
FT                   -> pNG18 7850bp
FT                   1. pNG18 remove PvuII-PvuII 520bp, Tn903 3' to kan
FT                   \ #V00359
FT                   -> pNG35 7330bp
FT                   1. YIp5 PvuII 5541bp 3250..3250
FT                   2. pNG35 PvuII-PvuII 1700bp, Tn903 kan gene/#V00359
FT                   -> YIp5-kanR 7200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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