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ID   YIP600     preliminary; circular DNA; SYN; 9460 BP.
AC   ATCC37471;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YIp600 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
RN   [2]
RC   from pCV13 & yeast genome
RC   pYC25B from pYC1 & yeast S288C genome
RC   YRp31 from pBR322 & pYC25B
RC   lambda CH2885 from lambda 1059 & yeast genome
RC   YIp333 from pBR322 & lambda CH2885, LYS2 gene
RC   YIp35C from YRp31 & URA3 gene
RA   Eibel H., Philippsen P.;
RT   "Identification of the cloned S. cerevisiae LYS2 gene by an
RT   integrative transformation approach";
RL   Mol. Gen. Genet. 191:66-73(1983).
RN   [3]
RC   pYC1 from HIS3 gene & yeast 2 micron & lambda, cos
RA   Hohn B., Hinnen A.;
RT   "Cloning with cosmids in E. coli and yeast";
RL   Genet. Eng. 2:169-183(1980).
CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp600)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YEp24 BamHI 7769bp 3785..3785
FT                   2. yeast BamHI-BamHI 10500bp, LYS2 gene
FT                   -> pDA6200 18269bp
FT                   1. pBR322 remove EcoRI-ClaI 26bp 4360..4361..25,
FT                   \ 4335bp
FT                   2. pDA6200 EcoRI-ClaI 4800bp, LYS2 gene
FT                   -> YIp600 9460bp [no ClaI]"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI-StuI-BglII-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;