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ID   YIP600     preliminary; circular DNA; SYN; 9460 BP.
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AC   ATCC37471;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp600 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
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RN   [2]
RC   from pCV13 & yeast genome
RC   pYC25B from pYC1 & yeast S288C genome
RC   YRp31 from pBR322 & pYC25B
RC   lambda CH2885 from lambda 1059 & yeast genome
RC   YIp333 from pBR322 & lambda CH2885, LYS2 gene
RC   YIp35C from YRp31 & URA3 gene
RA   Eibel H., Philippsen P.;
RT   "Identification of the cloned S. cerevisiae LYS2 gene by an
RT   integrative transformation approach";
RL   Mol. Gen. Genet. 191:66-73(1983).
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RN   [3]
RC   pYC1 from HIS3 gene & yeast 2 micron & lambda, cos
RA   Hohn B., Hinnen A.;
RT   "Cloning with cosmids in E. coli and yeast";
RL   Genet. Eng. 2:169-183(1980).
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CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp600)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 BamHI 7769bp 3785..3785
FT                   2. yeast BamHI-BamHI 10500bp, LYS2 gene
FT                   -> pDA6200 18269bp
FT                   1. pBR322 remove EcoRI-ClaI 26bp 4360..4361..25,
FT                   \ 4335bp
FT                   2. pDA6200 EcoRI-ClaI 4800bp, LYS2 gene
FT                   -> YIp600 9460bp [no ClaI]"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI-StuI-BglII-XhoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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