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ID   YRP14ARS1C preliminary; circular DNA; SYN; 11500 BP.
AC   L22015; M11994; M11997; M11995; M11996; M11998; M11999; ATCC77161;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YRp14/ARS1/CEN4 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   from YRp14/ARS1
RA   Hieter P., Pridmore D., Hegemann J.H., Thomas M., Davis R.W.,
RA   Philippsen P.;
RT   "Functional selection and analysis of yeast centromeric DNA";
RL   Cell 42:913-921(1985).
RN   [2]
RC   YRp14/CEN4 from YRp14 & CEN4 or pNN130
RC   YRp14/CEN4/ARS1 from YRp14/CEN4 & ARS1
RC   YRp14/CEN4/ARS2 from YRp14/CEN4 & ARS2
RC   YRp14/CEN4/H4-ARS from YRp14/CEN4 & H4 ARS
RC   YRp14/CEN4/H2B-ARS from YRp14/CEN4 & H2B ARS
RC   YRp14/CEN4/ARS4 from YRp14/CEN4 & ARS4
RC   YRp14/CEN4/ARS1 from YRp14/CEN4 & ARS1
RC   YRp14/CEN4/ARS1/ARS1 from YRp14/CEN4 & ARS1
RC   YRp14/ARS1 from YRp14 & ARS1, both EcoRI-HindIII 800bp
RC   YRp14/ARS1/CEN4 from YRp14/ARS1 & CEN4
RC   YRp14/ARS1/CEN3 from YRp14/ARS1 & CEN3
RC   [pSE271 from TRP1 gene]
RC   [p115 from SUP11 gene]
RA   Hieter P., Mann C., Snyder M., Davis R.W.;
RT   "Mititic stability of yeast chromosomes: a colony color assay
RT   that measures nondisjunction and chromosome loss";
RL   Cell 40:381-392(1985).
RN   [3]
RC   YRp14 or pNN59, YRp15 or pNN49 from YIp5 & SUP11-1 gene
RC   YRp15-galdelta series from YRp15 & GAL7/GAL10/GAL1 genes
RC   [YRp14-Sc2605 from YRp14]
RC   [YRp15-Sc494 from YRp15]
RA   St John T.P., Scherer S., McDonell M., Davis R.W.;
RT   "Deletion analysis of the Saccharomyces GAL gene cluster:
RT   transcription from three promoters";
RL   J. Mol. Biol. 152:317-334(1981).
CC   YC-type shuttle plasmid which can be used as a reporter of chromosome
CC   segregation due to loss of SUP11-containing chromosomes.
CC   Constructed by inserting a 0.8 kb EcoRI/HindIII fragment containing
CC   ARS1 and a 6.0 kb BamHI/SalI fragment containing CEN4 into the
CC   corresponding sites of YRp14.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI/HindIII--6.1, 2.3, 1.6, 0.8, 0.7; BamHI--11.5; EcoRI--5.5, 4.5,
CC   1.5; SalI--11.5. (ATCC staff)
CC   The order of the major features in this plasmid is: ARS1 - BamHI -
CC   CEN4 - SalI - URA3 - SUP11 - rep - pMB1 ori - bla.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YRp14/ARS1/CEN4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YRp14/ARS1 from YRp14 and ARS1)
CC   BR (YRp14/HIS3)(YRp14/TRP1)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YRp14 remove small EcoRI-HindIII
FT                   2. yeast EcoRI-HindIII 800bp, ARS1
FT                   -> YRp14/ARS1
FT                   1. YRp14/ARS1 remove small BamHI-SalI
FT                   2. yeast BamHI-SalI 6000bp, CEN4
FT                   -> YRp14/ARS1/CEN4"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT yeast SUP11 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;