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ID   YRP610     preliminary; circular DNA; SYN; 10900 BP.
AC   ATCC37472;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector YRp610 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   [pYE41(CEN3)10 from pBR322 & LEU2 gene & ARS1 & pYe(CEN3)11]
RC   pDA6200 from YEp24 & LYS2 gene
RC   YIp600, YIp601 from pBR322 & pDA6200
RC   YEp620, YEp620R from YIp600 & yeast 2 micron
RC   YRp610, YRp610R from YIp600 & YRp7
RC   YCp630, YCp631, YCp632 from YIp600 & pYE41(CEN3)10
RA   Barnes D.A., Thorner J.;
RT   "Genetic manipulation of Saccharomyces cerevisiae by use of the
RT   LYS2 gene";
RL   Mol. Cell. Biol. 6:2828-2838(1986).
CC   A general purpose replicating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YRp610)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. YIp600 EcoRI 9460bp 0..0
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS/TRP1 gene
FT                   -> YRp610 10906bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BstXI-MstII-XbaI-XhoI-StuI-HpaI-
FT                   BalI-AvaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;