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ID   YRP717CAMP preliminary; circular DNA; SYN; 6820 BP.
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AC   ATCC37630;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YRp7-17camp - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp7-32cat from YRp7 & pBR325
RC   YIp5-32cat from YRp7-32cat & YIp5
RC   YCp7-32cat from YRp7-32cat & pLA417
RC   [M13mp8::PHO5 from M13mp8 & PHO5]
RC   YRp7-32phocat from YRp7-32cat & M13mp8::PHO5
RC   YIp5-32phocat from YRp7-32phocat & pLA417
RC   YCp7-32phocat from YRp7-32phocat & pLA417
RC   YRp7-17camp from YRp7 & pBR325
RC   YRp8 from YRp7 & YIp5
RC   [M13mp10::CEN6 from M13mp10 & CEN6]
RC   YIp5CEN from YIp5 & M13mp10::CEN6
RC   YCp8 from YRp7 & YIp5CEN
RC   YRp8cat from YRp8 & YRp7-32cat
RC   YRp8phocat from YRp8 & YRp7-32phocat
RC   YCp8cat from YCp8 & YRp7-32cat
RC   YCp8phocat from YCp8 & YRp7-32phocat
RA   Mannhaupt G., Pilz U., Feldmann H.;
RT   "A series of shuttle vectors using chloramphenicol
RT   acetyltransferase as a reporter enzyme in yeast";
RL   Gene 67:287-294(1988).
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CC   One of a series (ATCC 37624 through 37634) of Escherichia
CC   coli/Saccharomyces cerevisiae shuttle vectors for investigating
CC   control of expression in yeast.
CC   A 5.47 kb ClaI/BamHI fragment of YRp7 was ligated to a 1.35 kb Sau96I
CC   fragment of pBR325.  Contains the complete bacterial cml gene.
CC   The depositor recommends using YRp7-17camp (ATCC 37630) as the
CC   reference for CAT expression and using the CAT assay methodology of
CC   Neumann, et al.
CC   Restriction digests of the clone give the following sizes (kb):
CC   SalI--7.0; EcoRI--4.6, 1.4, 0.82; PvuII--4.9, 2.05. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YRp7-17camp)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae SHY1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp7 remove TaqI-TaqI 313bp 270..583,
FT                   \ 5425bp
FT                   2. pBR325 Sau96I/AsuI-Sau96I/AsuI 1347bp 4343..5690,
FT                   \ cat gene
FT                   -> YRp7-17camp 6785bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   promoter        0..0
FT                   /note="PRO E. coli chloramphenicol resistance gene
FT                   (cat)(cml)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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