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ID   YRP732PCAT preliminary; circular DNA; SYN; 6530 BP.
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AC   ATCC37625;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YRp7-32phocat - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YRp7-32cat from YRp7 & pBR325
RC   YIp5-32cat from YRp7-32cat & YIp5
RC   YCp7-32cat from YRp7-32cat & pLA417
RC   [M13mp8::PHO5 from M13mp8 & PHO5]
RC   YRp7-32phocat from YRp7-32cat & M13mp8::PHO5
RC   YIp5-32phocat from YRp7-32phocat & pLA417
RC   YCp7-32phocat from YRp7-32phocat & pLA417
RC   YRp7-17camp from YRp7 & pBR325
RC   YRp8 from YRp7 & YIp5
RC   [M13mp10::CEN6 from M13mp10 & CEN6]
RC   YIp5CEN from YIp5 & M13mp10::CEN6
RC   YCp8 from YRp7 & YIp5CEN
RC   YRp8cat from YRp8 & YRp7-32cat
RC   YRp8phocat from YRp8 & YRp7-32phocat
RC   YCp8cat from YCp8 & YRp7-32cat
RC   YCp8phocat from YCp8 & YRp7-32phocat
RA   Mannhaupt G., Pilz U., Feldmann H.;
RT   "A series of shuttle vectors using chloramphenicol
RT   acetyltransferase as a reporter enzyme in yeast";
RL   Gene 67:287-294(1988).
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CC   A 570 bp SalI/EcoRI fragment containing the PHO5 promoter was ligated
CC   to a 5965 bp BamHI/SalI fragment of YRp7-32cat (ATCC 37624).
CC   The depositor recommends using YRp7-17camp (ATCC 37630) as the
CC   reference for CAT expression and using the CAT assay methodology of
CC   Neumann, et al.
CC   One of a series (ATCC 37624 through 37634) of Escherichia
CC   coli/Saccharomyces cerevisiae shuttle vectors for investigating
CC   control of expression in yeast.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.5, 1.37, 0.55; PstI--5.5, 1.3; PvuII--4.7, 2.15. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YRp7-32phocat)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp8 SmaI 7229bp 6239..6239
FT                   2. yeast BamHI-DraI 560bp, PHO5 promoter
FT                   -> M13mp8::PHO5 7789bp
FT                   1. YRp7-32cat BamHI-SalI 5965bp, YRp7 582..5738..306
FT                   2. M13mp8::PHO5 EcoRI-SalI 570bp, PHO5 promoter
FT                   \ M13mp8 6232..6239-PHO5-6240..6248
FT                   -> YRp7-32phocat 6535bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   promoter        0..0
FT                   /note="PRO yeast PHO5 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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