p219


Vector IG Sequence Link :
General : plasmid ds-DNA 8800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Saccharomyces cerevisiae)
Suppliers : (ATCC)
Misc.Comments : An EcoRI/HindIII double digest will give a 7.4 kb fragment of the vector retaining all the vector functions. The EcoRI site is just downstream of the yeast promoter. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--8.8; PstI--7.2, 1.6; HindIII--6.6, 2.2; XbaI--6.85, 1.95. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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