p366)(pBS32


Vector IG Sequence Link :
General : plasmid ds-DNA 8479 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Derived from YCp50 (ATCC 37419) by removing most of the URA3 gene as a 1.89 kb SalI/SmaI fragment and replacing it with a 2.23 kb SalI/XhoI fragment containing LEU2. (personal communication) YC-type shuttle vector. (personal communication) P. Hieter <-- J. Trueheart. (personal communication) The order of the major features in this plasmid is:HindIII - BamHI - SphI - LEU2 - CEN4 - ARS1 - pMB1 ori - bla. (personal communication) This vector was constructed by Josh Trueheart. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--6.5, 2.0; BamHI--8.5; HindIII--8.5; BglII--8.5; BamHI/BglII--5.6, 2.9. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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