pAA3.7X


Vector IG Sequence Link :
General : plasmid ds-DNA 9583 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Other pAA-vectors being precursors to pAA3.7X are in Pouwels A-I-i-1. [1] describes a new quick sequencing method, which involves the subdivision of the DNA fragment to be sequenced into many overlapping segments by a series of deletions which start from a fixed site. Since the different segments retain their natural connectedness, the complete sequence is easily assembled from overlaps that occur between the sequences of adjacent deletions. A simple and rapid method for the isolation of transposon-promoted deletions and their use in sequencing long DNA fragments was developed.
Parents : (PLMCG)(pBR322)(pBR325)(EcoGalK)(M11151)(NNLRSIS)(INS1ECLAC)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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