pAB366


Vector IG Sequence Link :
General : plasmid ds-DNA 4200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
Suppliers : (ATCC)
Misc.Comments : not original sequence. fusion regions. Shuttle vector conferring bleomycin and phleomycin resistance. The order of the major features in this plasmid is:pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - HindIII - pUC8. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7,1.5; BamHI/XhoI--3.4, 0.8; BglI--3.0, 1.2. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage