pAB366-2


Vector IG Sequence Link :
General : plasmid ds-DNA 4400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Differs from pAB366 (ATCC 77134) by the insertion of a NsiI cloning site in the 5' untranslated region of ble to permit transcriptional fusion. The order of the major features in this plasmid is:pTZ19R - EcoRI - BamHI - am promoter - NsiI - ble - XhoI - am 3' region - HindIII - pTZ19R. Shuttle vector conferring bleomycin and phleomycin resistance. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.9,1.5; BamHI/XhoI--3.6, 0.8; BglI--3.0, 1.4. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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