pAB366Ben


Vector IG Sequence Link :
General : plasmid ds-DNA 7200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Shuttle reporter plasmid to test expression of bleomycin resistance in transformants. [1] Expresses the mutant beta-tubulin gene conferring benomyl resistance. [1] A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions. [2] The order of the major features in this plasmid is:pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - tub-2 (benomyl resistance) - pUC8. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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