pAB520


Vector IG Sequence Link :
General : plasmid ds-DNA 4100 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Aspergillus nidulans)(Neurospora crassa)(E.coli)
Suppliers : (ATCC)
Misc.Comments : That expression was improved by modifying the 17 bp before the initiation codon in pAB366 to match the sequence of the am gene. Shuttle vector conferring bleomycin and phleomycin resistance. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. The am::ble fusion cassette can be removed by digestion with either HindIII, SphI, XbaI or BamHI or any combination of them. The order of the major features in this plasmid is:pUC18 - MCS (HindIII to BamHI) - am promoter - ble - XbaI - am 3' region - EcoRI - MCS (HindIII to EcoRI) - pUC18. Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.4; KpnI--4.1; SmaI--3.1, 1.0. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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