pAH-delta-N3


Vector IG Sequence Link :
General : plasmid ds-DNA 54 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (yeast AH22)
Suppliers : ()
Misc.Comments : clone pHP3 = rabbit minor cytochrome P-450 pAHF3 contained the entire coding sequence of pHP3 (RABP45HP3), but nucleotide sequences for the N-terminal region of pHP3 protein (from the 2nd to the 23rd amino acid) were deleted in pAH-delta-N3. On introduction into yeast AH22 cells, the absorption spectrum of cytochrome P-450 was detected in the microsomal fraction from the transformed cells carrying pAHF3. On the other hand, cytochrome P-450 could not be detected spectrophotometrically in any subcellular fractions from the yeast cells carrying pAH-delta-N3, although the transcript of pHP3 insert was detected in RNA blot analysis. NCBI gi: 220959
Parents : (pAAH5)(pHP3)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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