pAM34


Vector IG Sequence Link :
General : plasmid ds-DNA 6000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JS219)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Permits positive selection because deletion of the aadA fragment is not viable. pAM35 (ATCC 77186) differs from pAM34 (ATCC 77185) by deletion of the DraII fragment in pAM34 carrying lacIq. The order of the major features in this plasmid is: lacZpo - ori - rom - transcription terminator- BamHI - MCS - XmaIII - aadA (spcR) - MCS - BamHI - transcription terminator - bla - lacIq. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; BamHI--4.3,1.8; HindIII--6.0; PstI--6.0; KpnI--3.4, 2.7. (ATCC staff) Medium is -1 1227 + IPTG (1 mM).
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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