pARO191


Vector IG Sequence Link :
General : plasmid ds-DNA 6070 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(Enterobacter aerogenes)(E.coli)(E.coli S17-1 [ATCC 47055])
Suppliers : (ATCC)
Misc.Comments : Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Mobilizable expression vector encoding the lacZalpha (lacZ') peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Replicates to high copy number in E.coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. The order of the major features in this plasmid is:lacZ'/MCS - pMB1 ori - kanR - oriT/bom. pARO181 (ATCC 77125) and pARO191 (77126) differ in the orientation of the MCS in lacZ'. Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, EcoRI/HindIII--6.0; PstI--3.8, 2.2; BamHI--6.0; KpnI--6.0. (ATCC staff) Medium is 1236 LB plus kanamycin.
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