pAS2


Vector IG Sequence Link :
General : phagemid ds-DNA 8500 BP
Functions : (cloning)(expression)(shuttle - episomal)(DNA library
Selection : (CYH2 ribosomal protein L29)
Copy Number :
Hosts : (E.coli)(yeast)(Saccharomyces cerevisiae)
Suppliers : (CLONTECH)(ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. [1] The order of the major features in this plasmid is: ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR - 2 micron ori - TRP1 -> - f1 ori - <- CYH2. [2] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J.
Parents : ()
Siblings : ()
Descendents : ()


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