pASZ10


Vector IG Sequence Link :
General : phagemid ds-DNA 5396 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The ADE2 segment contains HindIII sites approximately 1.17 kb and 2.36 kb from the 5' BglII site. The naturally-occurring BglII site within ADE2 was destroyed by replacing the A at nt 1243 of the reported sequence with a G. A BglII linker was added to the 5' end. and the naturally-occurring BglII site at the 3' end (nt 2512) was used to create a BglII fragment containing the complete coding sequence. Constructed by inserting a 2.5 kb fragment (Ars+ Ade2+) into the BglII site of pTZ19B. A YR-type shuttle expression vector containing promoters for in vitro RNA synthesis and priming sites useful for sequencing. Contains readily purifiable elements for construction experiments. In E.coli, permits visual detection of recombinants and production of ssDNA. Encodes the lacZalpha (lacZ') peptide. pTZ19B was derived from pTZ19R by adding a BglII linker at coordinate 416. The order of the major features in this plasmid is:lacZ'/MCS - T7 promoter - ADE2 - pMB1 ori - ampR - f1 ori. Restriction digests of the clone give the following sizes (kb): BglII--2.9, 2.6; EcoRI--5.6; HindIII--2.9, 1.3, 1.1, 0.41; BamHI--5.6. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
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Descendents : ()

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