pAU21


Vector IG Sequence Link :
General : plasmid ds-DNA 5300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Schizosaccharomyces pombe)(E.coli)(Schizosaccharomyces pombe [ATCC 38436])
Suppliers : (ATCC)
Misc.Comments : Shares pBR322 sequences from the PvuII site to the ScaI site with pcD. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Contains the ars sequence from Schizosaccharomyces pombe and the URA3 gene from Saccharomyces cerevisiae. Insertions at the BamHI or SalI sites inactivate tetracycline resistance. Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--2.6 (doublet); PstI--5.2. (ATCC staff) Medium is 1273 LB plus tetracycline.
Parents : ()
Siblings : ()
Descendents : ()


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